Abstract: Objective To understand the awareness of AIDS related knowledge, prevalence of risk behaviors and related factors, infection status of HIV, syphilis and HCV among female sex workers (FSWs) in Shaoxing and provide evidence for the comprehensive prevention and control of AIDS. Methods According to the national AIDS sentinel surveillance protocol (2012 edition), questionnaire survey and serological detection were conducted among FSWs in six counties of Shaoxing from April to June,2013. Results Totally 2423 FSWs were surveyed in 2013. The HIV, syphilis infection rates were 0.04% and 1.9% respectively, but no HCV positive FSWs were detected. The awareness rate of AIDS related knowledge was 90.7%, but only 76.1% of FSWs knew whether mosquito can transmit HIV or not. Multivariate logistic regression analysis indicated that the awareness of AIDS related knowledge was associated with the age, grade, educational level and local residence duration of the FSWs, but the grade specific differences in receiving health education, peer advise and HIV detection during the past year among FSWs were statistical significant(2=9.336,P=0.009; 2=59.513,P0.001; 2=8.032,P=0.018). Conclusion Older age, low grade, low educational level and short local residence were closely related with low awareness of AIDS related knowledge in FSWs in Shaoxing, it is necessary to conduct targeted AIDS prevention and control among FSWs.