Abstract: Objective To compare the quality of diagnosis and reporting of hepatitis B in Nanan, Jinjiang and Anxi counties in Quanzhou, Fujian province before (2011) and after (2013) hepatitis B surveillance program implementation. Methods Reported hepatitis B cases seeking medical care in 9 medical institutions in the 3 counties in 2011 and 2013 were selected by stratified random sampling to evaluate the related diagnosis and reporting according to the medical records and laboratory detection results. Questionnaire survey was conducted among the doctors in the 9 medical institutions to understand their awareness of hepatitis B diagnosis and reporting. Results The accurate rates of hepatitis B reporting were 93.4% (255/273) and 96.7% (265/274), respectively, in 2011 and 2013,the mis-reporting rates were 6.6% (18/273) and 3.3% (9/274), respectively and the consistency rates in hepatitis B diagnosis classification were 78.1% and 91.8%, respectively. Among 64 doctors surveyed, 14.1% said that hepatitis B cases should be reported repeatedly, and 3.1% believed that hepatitis B virus carrier should be reported too. In 2013,the reported incidence of hepatitis B was 67.5/lakh, a decline of 5.9% compared with 2011. The incidences of acute hepatitis B and unclassified cases declined by 82.9% and 85.8%. Conclusion The accuracy of hepatitis B reporting was improved obviously and incidence of acute hepatitis B declined significantly in the 3 counties. Because there were re-reporting, mis-reporting and misdiagnosis, it's necessary to strengthen the training and supervision on hepatic B diagnosis and reporting.