Abstract: Objective To understand the genotypes of beta-lactamases carried by carbapenems resistant Enterobacteriaceae (CRE) isolated from sick children in our hospital and the susceptibility of CRE to antibiotics. Methods The identification and drug susceptibility test of the strains were performed with VITEK compact-2 automatic system. Carbapenemase was detected by using Modified Hodge test. -lactamase genes, including blaKPC, blaTEM, blaSHV, blaIMP-1, blaIMP-2, blaVIM-1, blaVIM-2, blaCTXM-1, blaCTXM-2, blaCTXM-9 and blaNDM-1, were detected by PCR and confirmed by sequencing. Results A total of 13 strains of CRE were collected from January 1, 2010 to December 31, 2012. All the strains were resistant to -lactams, -lactamase inhibitor. The resistant rate to cotrimoxazole was 100%, the resistance rate to tobramycin was 15.38% and the resistant rate to amikacin was 7.69%. PCR results indicated that all the strains carried blaTEM gene, 11 strains (84.62%) carried blaKPC gene, and the detection rates of blaSHV, blaCTXM-1, blaCTXM-9 and blaVIM-1.genes were 46.15%, 46.15%, 15.38% and 7.69%, respectively. Conclusion Klebsieila pneumoniae carbapenemase producing was the main reason for CRE resistance to carbapenems in our hospital. Most CRE strains were multi drug resistant. Amikacin can be the first choice for treatment of CRE infection.