北京市丰台区诺如病毒暴发疫情的实验室鉴定及基因特征分析[J]. 疾病监测, 2015, 30(2): 118-121. DOI: 10.3784/j.issn.1003-9961.2015.02.009
引用本文: 北京市丰台区诺如病毒暴发疫情的实验室鉴定及基因特征分析[J]. 疾病监测, 2015, 30(2): 118-121. DOI: 10.3784/j.issn.1003-9961.2015.02.009
Etiological identification and genotyping of norovirus detected in gastroenteritis outbreaks in Fengtai, Beijing[J]. Disease Surveillance, 2015, 30(2): 118-121. DOI: 10.3784/j.issn.1003-9961.2015.02.009
Citation: Etiological identification and genotyping of norovirus detected in gastroenteritis outbreaks in Fengtai, Beijing[J]. Disease Surveillance, 2015, 30(2): 118-121. DOI: 10.3784/j.issn.1003-9961.2015.02.009

北京市丰台区诺如病毒暴发疫情的实验室鉴定及基因特征分析

Etiological identification and genotyping of norovirus detected in gastroenteritis outbreaks in Fengtai, Beijing

  • 摘要: 目的 对北京市丰台区2014年5-6月发生的3起诺如病毒急性胃肠炎暴发疫情进行病原体鉴定及基因分型。方法 采用实时荧光定量-聚合酶链反应法对62份病例标本进行诺如病毒核酸检测,阳性标本经反转录-聚合酶链反应扩增RNA多聚酶区部分序列,使用BioEdit及Mega 6.0软件对扩增序列进行同源性及进化分析。结果 36份标本中检出GⅡ型诺如病毒,阳性率为58.06%;序列分析表明幼儿园为GⅡ.7型,学校1为GⅡ.8型,学校2为GⅡ.6型。结论 诺如病毒是引起丰台区急性胃肠炎暴发疫情的主要病原体,丰台区流行的诺如病毒基因型存在多样性。

     

    Abstract: Objective To identify the pathogens causing 3 gastroenteritis outbreaks during May-June 2014 in Fengtai, Beijing and detect genotypes of the pathogens. Methods A total of 62 samples were collected from the cases to detect norovirus by using real-time fluorescent quantitative PCR. The positive samples were amplified by using one-step RT-PCR, then the amplification products were sequenced for nucleotide sequence alignments and phylogenetic analysis with BioEdit and Mega 6.0 software. Results Thirty six samples were norovirus GⅡ positive (58.06%).The alignment and phylogenetic analysis revealed that the outbreak in child care setting was caused by norovirus GⅡ.7, the outbreak in school 1 was caused by norovirus GⅡ.8 and the outbreak in school 2 was caused by norovirus GⅡ.6. Conclusion Norovirus was the pathogen causing the gastroenteritis outbreaks in Fengtai, and multi genotype norovirus co-circulated in Fengtai.

     

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