肺炎克雷伯菌泛耐药株的质粒耐药元件研究[J]. 疾病监测, 2015, 30(2): 134-139. DOI: 10.3784/j.issn.1003-9961.2015.02.013
引用本文: 肺炎克雷伯菌泛耐药株的质粒耐药元件研究[J]. 疾病监测, 2015, 30(2): 134-139. DOI: 10.3784/j.issn.1003-9961.2015.02.013
Resistant elements in plasmid of extensive drug resistant Klebsiella pneumonia[J]. Disease Surveillance, 2015, 30(2): 134-139. DOI: 10.3784/j.issn.1003-9961.2015.02.013
Citation: Resistant elements in plasmid of extensive drug resistant Klebsiella pneumonia[J]. Disease Surveillance, 2015, 30(2): 134-139. DOI: 10.3784/j.issn.1003-9961.2015.02.013

肺炎克雷伯菌泛耐药株的质粒耐药元件研究

Resistant elements in plasmid of extensive drug resistant Klebsiella pneumonia

  • 摘要: 目的 通过对泛耐药肺炎克雷伯菌JM45的质粒1(pJM45-1)耐药元件分析,从基因组水平研究其泛耐药的遗传学基础。方法 采用第二代高通量测序技术平台进行全基因组测序,生物信息学方法分析pJM45-1质粒的耐药元件,并与已在NCBI登录的质粒序列作聚类分析(Fast Minimum Evolutin法)。结果 pJM45-1质粒大小为317 156 bp,功能注释分析发现该质粒为可接合转移质粒,携带了抗菌药物耐药编码基因、重金属离子耐受编码基因、毒素编码基因和转座子以及插入序列等83个耐药相关编码基因。pJM45-1质粒与耐药质粒R100(登录号:AP000342.1)的全长94 281个序列中的45 995个序列有99%相同;与接合性质粒F质粒(登录号:AP001918.1)的全长99 159个序列中的8322个序列有87%相同。pJM45-1质粒与源自肺炎克雷伯菌的质粒在同一簇(cluster),与源自其他肠杆菌的质粒不在一个簇。结论 肺炎克雷伯菌JM45 pJM45-1质粒携带大量耐药元件,是该菌株进化为泛耐药的主要原因。pJM45-1是可接合转移质粒,可将耐药基因在细菌间进行水平转移,造成耐药菌的播散。

     

    Abstract: Objective To understand the genetics mechanism of extensive drug resistant Klebsiella pneumoniae by analyzing its resistant elements in plasmid pJM45-1. Methods Whole genome sequencing for an extensive drug resistant K. pneumoniae strain was performed by using high throughput Roche 454 platform, then the resistant elements in its plasmid pJM45-1 were analyzed, and the cluster analysis was performed by using the sequences of the plasmids recorded by National Center of Biotechnology Information (NCBI) with Fast Minimum Evolution method. Results The length of plasmid pJM45-1 was 317 156 bp. The encoding genes of transfer genes of conjuagal plasmids, resistant genes to antibiotics and heavy metal ions, virulence factors, transposons and insertion sequences were detected in plasmid pJM45-1. Moreover, plasmid pJM45-1 was 99% identical to 45 995 bases in 94 281 bases of resistant plasmid R100 (Genbank accession No.AP000342.1), while 87% identical to 8322 bases in 99 159 bases of F plasmid (Genbank accession No.AP001918.1). So plasmid pJM45-1 might be a chimeric plasmid. In addition, plasmid pJM45-1 and plasmids in other K. pneumoniae isolates were in the same cluster, while plasmid pJM45-1 and plasmids in other enterobacteria isolates were in the different cluster. Conclusion The resistant genes to antibiotics and heavy metal ions in plasmid pJM45-1 played a key role in the development of the extensive drug resistance of K. pneumoniae. Furthermore, plasmid pJM45-1 is a conjugative transfer plasmid, and resistance genes could be horizontally transferred among bacteria to cause the spread of drug-resistant strains.

     

/

返回文章
返回