Abstract: Objective To investigate the distribution and drug resistance of Staphylococcus in patients with urinary tract infection in our hospital, and provide evidence for the rational use of antibiotics in hospitals. Methods The urine specimens collected from January 2007 to December 2013 were cultured in Uricult, VITEK-60 automatic analyzer was used for strain identification and drug susceptibility test, and a retrospective analysis was carried out on the urine culture results with software WHONET 5.6. Results A total of 284Staphylococcus strains were detected, in which 118 Staphylococcus epidermidis(41.5%), 49 Staphylococcus haemolyticus(17.3%) and 40 Staphylococcus aureus (14.1%) ranked first to third. The Staphylococcus strains were mainly detected in internal medicine department (106 strains, 37.3%), followed by urology department (89 strains, 31.3%). The resistance rates of staphylococcus were high to penicillin, ampicillin/sulbactam, oxacillin and erythromycin (73.3%), and low to linezolid, vancomycin, quinupristin/dalfopristin, rifampin, nitrofurantoin and moxifloxacin (0.0%-16.4%). The detection rate of MRS was 75.9%. Compared with 2007, the resistant rates to moxifloxacin and clindamycin increased obviously in 2013, but the resistant rates to oxacillin, gentamicin, levofloxacin, erythromycin, sulfamethoxazole and tetracycline declined. Conclusion Staphylococcus epidermidis was major pathogen to cause with urinary tract infection; Nitrofurantoin is suitable for experiential treatment of urinary tract infection. It is important to regularly monitor the distribution and drug resistance of the pathogens causing urinary tract infection in order to guide clinical antibiotic use.