浙江省绍兴市46例男男性行为者HIV感染基因特征分析及流行病相关因素分析

Genotyping of HIV-1 detected in HIV infected men who have sex with men in Shaoxing, Zhejiang

摘要: 目的了解浙江省绍兴市男男性行为者(MSM)人群中HIV-1感染者的亚型类型和特征。方法分析2013年1月至2014年7月本地区新确证未经抗病毒治疗的50例MSM感染者样本,采用RT-PCR和nest-PCR方法扩增HIV-1的gag和pol全长基因区,成功扩增46份样品,构建系统进化树分析基因亚型。结果 46份样品中发现CRF01_AE、CRF07_BC和B三种亚型和1株AE、BC重组亚型,各占52.17%、39.13%、6.52%和2.17%。结论绍兴市 HIV-1感染的MSM人群中主要流行的亚型为CRF01_AE和CRF07_BC,该人群感染率高,应加强监测。

Abstract: Objective To understand the genotypes of HIV-1 circulating in HIV infected men who have sex with men(MSM) in Shaoxing, Zhejiang province.Methods Fifty blood samples were collected from 50 newly-diagnosed HIV infected MSM who received no antiviral therapy from 2013 to 2014,and the gag and pol gene fragments of HIV-1 were amplified with RT-PCR and nest-PCR. The gene phylogenetic tree was constructed to analyze the subtypes of HIV strains.Results A total of 46 plasma samples were successfully amplified and analyzed.Three HIV subtypes were detected, i.e. CRF01_AE, CRF07_BC and B, accounting for 52.17%, 39.13% and 6.52%. In addition, 1 new recombinant subtype was detected(2.17%).Conclusion CRF01_AE and CRF07_BC were the major HIV-1 subtypes detected among MSM in Shaoxing. It is necessary to strengthen the surveillance of HIV-1 subtype CRF01_AE and CRF07_BC in MSM.