王欣儒, 王建平, 罗霞, 孙强正. 福氏志贺菌gtrI基因突变导致的血清型回复转换[J]. 疾病监测, 2015, 30(12): 1024-1027. DOI: 10.3784/j.issn.1003-9961.2015.12.009
引用本文: 王欣儒, 王建平, 罗霞, 孙强正. 福氏志贺菌gtrI基因突变导致的血清型回复转换[J]. 疾病监测, 2015, 30(12): 1024-1027. DOI: 10.3784/j.issn.1003-9961.2015.12.009
WANG Xin-ru, WANG Jian-ping, LUO Xia, SUN Qiang-zheng. Serotype conversion due to gtrI gene dysfunctional mutation in Shigella flexneri serotype Y strains[J]. Disease Surveillance, 2015, 30(12): 1024-1027. DOI: 10.3784/j.issn.1003-9961.2015.12.009
Citation: WANG Xin-ru, WANG Jian-ping, LUO Xia, SUN Qiang-zheng. Serotype conversion due to gtrI gene dysfunctional mutation in Shigella flexneri serotype Y strains[J]. Disease Surveillance, 2015, 30(12): 1024-1027. DOI: 10.3784/j.issn.1003-9961.2015.12.009

福氏志贺菌gtrI基因突变导致的血清型回复转换

Serotype conversion due to gtrI gene dysfunctional mutation in Shigella flexneri serotype Y strains

  • 摘要: 目的 研究携带O抗修饰基因gtrI的福氏志贺菌Y血清型菌株特征。方法 采用血清型多重聚合酶链反应(PCR)分型方法检测携带gtrI基因但I型抗原表位阴性的福氏志贺菌Y血清型菌株,并对其gtrI基因簇进行序列分析,对其基因组进行脉冲场凝胶电泳(PFGE)分析。结果 5株福氏志贺菌基因组携带gtrI基因簇,但是I型抗血清凝集阴性,表现为Y血清型特征;测序发现这5株菌株中的gtrI基因均发生了功能失活性突变;PFGE分析显示这5株菌株与福氏志贺菌血清型1a菌株具有相同或相近的PFGE带谱。结论 携带O抗修饰基因gtrI的福氏志贺菌Y血清型菌株来源于血清型1a。

     

    Abstract: Objective To characterize the Shigella flexneri serotype Y strains carrying dysfunctional gtrI gene. Methods Multiple-PCR was conducted to screen S. flexneri strains which carry gtrI gene within genomes, but have antigenic epitope I on cell surface. The gtrⅠ gene clusters in these candidates were sequenced. The genome DNA of these strains were analyzed with PFGE and compared with that of other strains. Results A total of 5 strains were found to carry gtrI gene cluster within genomes, but not to react with typing I antisera, presenting the characteristics of serotype Y. Sequencing analysis suggested that all the strains had dysfunctional mutation in the gtrI gene sequence. The PFGE patterns of the 5 strains were identified, which were similar to that of serotype 1a strains. Conclusion The five serotype Y strains which carried dysfunctional gtrI gene were originated from serotype 1a through gtrI gene mutation.

     

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