内蒙古鼠疫耶尔森菌差异区段分型特征研究

蒋羽; 李建云; 梁莹; 蔡虹; 彭遥; 王宇萌; 刘芳; 徐冬蕾; 王姝懿; 张志凯; 海荣; 张忠兵; 李伟; 范蒙光

doi:10.3784/j.issn.1003-9961.2016.05.013

内蒙古鼠疫耶尔森菌差异区段分型特征研究

Different region genotyping of Yersinia pestisin Inner Mongolia

摘要

摘要: 目的研究分离自内蒙古不同鼠疫疫源地、不同宿主和媒介的鼠疫耶尔森菌的差异区段基因型分布特征。方法选取内蒙古3个鼠疫疫源地分离的鼠疫耶尔森菌307株,采用差异区段分析法,设计24对引物进行DFR位点扩增分型,用BioNumerics软件分析。结果通过差异区段分型方法共发现13种基因型,其中5种基因型(Gnm1~Gnm5)为本研究首次报道。长爪沙鼠疫源地有9种基因型,主要基因型为G11(71.8%,122/170);达乌尔黄鼠疫源地有6种基因型,主要为G10(44.1%,41/93)和G11(35.5%,33/93);布氏田鼠疫源地有4种基因型,主要为G14(90.9%,40/44)。3个疫源地均分布有G11型。结论内蒙古鼠疫耶尔森菌差异区段基因型分布具有明显的地理分布特征,对分析内蒙古鼠疫的传播和遗传演变具有重要的分子流行病学意义。

Abstract: Objective To study the geographic distribution of different region genotypes of Yersinia pestis isolated from different plague foci, hosts and media in Inner Mongolia. Methods A total of 307 strains isolated from three plague foci were selected for regional genotyping by using primers for 24 loci and software BioNumerics. Results A total of 13 genotypes were found, including 5 new genotypes(Gnm1-Gnm5). Nine genotypes, including the main genotype G11 (71.8%, 122/170), were found in Merionesunguiculatus plague focus in Inner Mongolia, and 6 genotypes were found in Spermophilus Dauricus plague focus in Songliaoplain, G10 (44.1%, 41/93) and G11 (35.5%, 33/93) accounted for high proportion. There were 4 genotypes in Lasiopodomysbrandtii plague focus in Xilingol grassland, G14 was predominant (90.9%, 40/44). Conclusion The geographic distribution of different region genotypes of Y. pestis was obvious in Inner Mongolia, the results has epidemiologic importance in analyzing the spread of Y. pestis and its genetic evolution in Inner Mongolia.

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