Abstract: Objective To understand the drug resistance of Mycobacterium(M.) tuberculosis in some areas of Jiangxi province and provide evidence for tuberculosis (TB) prevention and control and related evaluation. Methods The sputum samples collected from the active TB patients surveyed were used to culture M. tuberculosis for the drug susceptibility test. Results The overall drug resistant rate of M. tuberculosis was 27.21%,and the drug resistant rate was 23.21% in newly detected patients and 47.83% in retreat patient. The single drug resistant rate to isoniazid was highest (12.01%), and multi-drug resistance was detected in 11 cases (3.89%). Conclusion The standardized TB treatment and management has achieved initial success,but it is still necessary to further strengthen the surveillance for drug resistant TB.