徐小峰, 张仁庆, 马月, 吕燕, 黎建乐, 石强, 肖康, 孙静, 杨晓东, 董小平, 陈操. 羊瘙痒因子139A感染引起小鼠脑组织中的1-ACT表达增加[J]. 疾病监测, 2017, 32(4): 282-286. DOI: 10.3784/j.issn.1003-9961.2017.04.007
引用本文: 徐小峰, 张仁庆, 马月, 吕燕, 黎建乐, 石强, 肖康, 孙静, 杨晓东, 董小平, 陈操. 羊瘙痒因子139A感染引起小鼠脑组织中的1-ACT表达增加[J]. 疾病监测, 2017, 32(4): 282-286. DOI: 10.3784/j.issn.1003-9961.2017.04.007
XU Xiao-feng, ZHANG Ren-qing, MA Yue, LYU Yan, LI Jian-le, SHI Qiang, XIAO Kang, SUN Jing, YANG Xiao-dong, Dong Xiao-ping, CHEN Cao. Remarkable up-regulation of 1-antichymotrypsin in brains of scrapie agent 139A infected mice[J]. Disease Surveillance, 2017, 32(4): 282-286. DOI: 10.3784/j.issn.1003-9961.2017.04.007
Citation: XU Xiao-feng, ZHANG Ren-qing, MA Yue, LYU Yan, LI Jian-le, SHI Qiang, XIAO Kang, SUN Jing, YANG Xiao-dong, Dong Xiao-ping, CHEN Cao. Remarkable up-regulation of 1-antichymotrypsin in brains of scrapie agent 139A infected mice[J]. Disease Surveillance, 2017, 32(4): 282-286. DOI: 10.3784/j.issn.1003-9961.2017.04.007

羊瘙痒因子139A感染引起小鼠脑组织中的1-ACT表达增加

Remarkable up-regulation of 1-antichymotrypsin in brains of scrapie agent 139A infected mice

  • 摘要: 目的 探究1-ACT在羊瘙痒因子139A感染小鼠脑组织中的变化情况。方法 利用蛋白免疫印迹、免疫组织化学、间接免疫荧光及荧光共聚焦方法分析羊瘙痒因子139A感染小鼠脑组织中1-ACT表达的变化和分布特点。结果 蛋白免疫印迹方法显示在羊瘙痒因子139A感染小鼠终末期脑组织中1-ACT的含量较正常对照小鼠明显上调且随着潜伏期的延长而逐渐增加;免疫组织化学方法发现1-ACT主要分布于羊瘙痒因子139A感染小鼠的皮层、丘脑和小脑区域;间接免疫荧光实验显示羊瘙痒因子139A感染终末期小鼠脑组织中补体成分C3含量明显增加,同时荧光共聚焦实验表明1-ACT与补体成分C3存在明显的共定位现象。结论 羊瘙痒因子139A感染终末期小鼠脑组织中1-ACT含量明显增加。

     

    Abstract: Objective To understand the potential alterations of 1-antichymotrypsin (ACT) levels in the brains of scrapie agent 139A infected mice. Methods By using Western blot, immunohistochemistry assay, indirect immunofluorescent assay and confocal microscope assays, we analyzed the characteristics of the expressions and distributions of 1-ACT in the brains of scrapie agent 139A-infected mice. Results Compared with the normal controls, 1-ACT-specific Western blot showed that the levels of 1-ACT were remarkably up-regulated in the brains of scrapie-infected mice in a time-dependent manner during the incubation period after prion infection. Immunohistochemistry assay showed that the brain 1-ACT were mainly distributed in the regions of cortex, thalamus and cerebellum. Indirect immunofluorescent assay showed large amounts of complement C3 positive signals in the brain slices of 139A-infected mice, and confocal microscope assays further found brain 1-ACT to be colocalized well with complement C3. Conclusion Remarkable up-regulation of 1-ACT in the brains of scrapie agent 139A infected mice was observed at end stage of the infection.

     

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