Abstract: Objective To understand the factors influencing the incidence of congenital heart diseases (CHD) in Wenzhou and provide scientific evidence for the etiological study and prevention of CHD.Methods In this 1:1 case-control study,questionnaire survey was conducted to collect the information of mothers of 500 infants with CHD and 500 healthy infants during 2015-2016,and univariate and multivariate logistic regression analyses were conducted to identify CHD related factors.Results The univariate and multivariate logistic regression analyses showed that education level of college or above,singleton pregnancy,folic acid supplement were the protective factors,the odds ratios were 0.741(95%CI:0.551-0.996),0.191(95%CI:0.083-0.441) and 0.528(95%CI:0.368-0.757) respectively,exposure to chemicals,catching cold in early pregnancy,taking ovulation stimulants,exposure to pets,house decoration,hair dye,alcohol use,abnormal reproductive history,husband smoking were the risk factors,the odds ratios were 9.033(95%CI:1.116-73.129),1.616(95%CI:1.193-2.189),17.333(95%CI:7.197-136.751),2.679(95%CI:1.089-6.593),15.240(95%CI:5.945-39.068),3.030(95%CI:1.656-5.546),8.448(95%CI:1.043-68.432),3.028(95%CI:1.655-5.538) and 1.336(95%CI:1.008-1.772) respectively.Conclusion It is necessary to conduct active prevention and targeted intervention to reduce the incidence of CHD and improve population quality.