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亚红祥, 沈姝, 苏正元, 邓菲, 张云智. 云南省西北地区家畜体表蜱类形态学与分子生物学鉴定[J]. 疾病监测, 2017, 32(7): 609-614. DOI: 10.3784/j.issn.1003-9961.2017.07.019
引用本文: 亚红祥, 沈姝, 苏正元, 邓菲, 张云智. 云南省西北地区家畜体表蜱类形态学与分子生物学鉴定[J]. 疾病监测, 2017, 32(7): 609-614. DOI: 10.3784/j.issn.1003-9961.2017.07.019
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YA Hong-xiang, SHEN Shu, SU Zheng-yuan, DENG Fei, ZHANG Yun-zhi. Morphological and molecular identification of tick species from body surface of domestic animals in northwestern Yunnan[J]. Disease Surveillance, 2017, 32(7): 609-614. DOI: 10.3784/j.issn.1003-9961.2017.07.019
Citation: YA Hong-xiang, SHEN Shu, SU Zheng-yuan, DENG Fei, ZHANG Yun-zhi. Morphological and molecular identification of tick species from body surface of domestic animals in northwestern Yunnan[J]. Disease Surveillance, 2017, 32(7): 609-614. DOI: 10.3784/j.issn.1003-9961.2017.07.019
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云南省西北地区家畜体表蜱类形态学与分子生物学鉴定

Morphological and molecular identification of tick species from body surface of domestic animals in northwestern Yunnan

    摘要
  • 摘要: 目的 调查云南省西北地区家畜体表蜱的种类及其种群遗传进化情况。方法 采集家畜体表寄生的蜱虫，经形态学鉴定后，用PCR法扩增蜱样本的16S rDNA和ITS2基因片段，测序后进行序列分析。结果 共采集成蜱样本1 275只，经形态学鉴定为1科3属4种，其中微小扇头蜱1 263只（占99.1%），卵形硬蜱7只（占0.6%），锐跗硬蜱4只（占0.3%），未知血蜱1只（占0.1%）。样品分子鉴定结果与形态学鉴定结果一致。16S rDNA序列分析显示，蜱P6与印度微小扇头蜱（EU918188）的相似性最高为99.8%，与中国云南微小扇头蜱（JX051062）的相似性99.4%；蜱P2与美国卵形硬蜱（U95900）的相似性最高为93.8%；蜱P1与日本锐跗硬蜱（AB105167）的相似性最高为95.9%；蜱P4与澳大利亚parva血蜱（JX573136）的相似性为90.5%，与中国云南长角血蜱（JX051064）的相似性为88.7%。ITS2序列分析显示，蜱P6与来自老挝（KC503276）、中国云南（KC203364）的微小扇头蜱的相似性均为99.9%；蜱P2与日本卵形硬蜱（D88857）的相似性最高为96.1%；蜱P1与日本锐跗硬蜱（AB605168）的相似性最高为95.3%；蜱P4与罗马尼亚Haemaphysalis parva血蜱（FN296282）的相似性最高为91.0%。结论 云南地区家畜体表存在微小扇头蜱、卵形硬蜱、锐跗硬蜱和一种与parva血蜱相关的新型血蜱。

     

    Abstract: Objective To investigate the species composition and genetic variants of ticks in the northwest area of Yunnan province. Methods The collected ticks from the body surface of domestic animals were identified morphologically. The 16S rDNA and ITS2 segments of ticks were amplified respectively by PCR and sequenced. The homologies of the above segments with other known sequences were analyzed. Results A total of 1 275 ticks were collected from the body surface of domestic animals and classified into four species of three genus of one family by species morphological identification. Of them, 1 263 (99.1%) were Rhipicephalus microplus, 7 (0.6%) were Ixodes ovatus, 4 (0.3%) were Ixodes acutitarsus and 1 (0.1%) were Haemaphysalis sp. Molecular detection confirmed the results of the morphological identification. Sequence analysis showed that 16S rDNA fragments of tick P6 shared highest homology (99.8%) with Rhipicephalus microplus (EU918188) from India, and 99.4% homology with Rhipicephalus microplus (JX051062) from Yunnan of China. 16S rDNA fragments of tick P2's shared highest homology (93.8%) with Ixodes ovatus (U95900) from USA. Tick P1's 16S rDNA fragments shared highest homology (95.9%) with Ixodes acutitarsus (AB105167) from Japan. Tick P4's 16S rDNA fragments shared highest homology (90.5%) with Haemaphysalis parva (JX573136) from Australia, and 88.7% homology with Haemaphysalis longicornis (JX051064) from Yunnan of China. Tick P6's ITS2 fragments shared highest homology (99.9%) with Rhipicephalus microplus from Laos (KC503276) and Yunnan of China (KC203364). Tick P2's ITS2 fragments shared highest homology (96.1%) with Ixodes ovatus (D88857) from Japan. Tick P1' ITS2 fragments shared highest homology (95.3%) with Ixodes acutitarsus (AB605168) from Japan. Tick P4's ITS2 fragments shared highest homology (91.0%) with Haemaphysalis parva(FN296282)from Romania. Conclusion Rhipicephalus microplus, Ixodes ovatus, Ixodes acutitarsus and a new Haemaphysalis sp. which is related to Haemaphysalis parva, were detected on the body surface of domestic animal in Yunnan province.

     

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