杨卫红, 宋秀平, 梁伟, 冯云, 章域震, 张云智, 张海林, 栗冬梅. 云南省德宏州居住区啮齿动物携带巴尔通体的调查研究[J]. 疾病监测, 2018, 33(1): 20-25. DOI: 10.3784/j.issn.1003-9961.2018.01.006
引用本文: 杨卫红, 宋秀平, 梁伟, 冯云, 章域震, 张云智, 张海林, 栗冬梅. 云南省德宏州居住区啮齿动物携带巴尔通体的调查研究[J]. 疾病监测, 2018, 33(1): 20-25. DOI: 10.3784/j.issn.1003-9961.2018.01.006
Yang Weihong, Song Xiuping, Liang Wei, Feng Yun, Zhang Yuzhen, Zhang Yunzhi, Zhang Hailin, Li Dongmei. Investigation of natural infection status of Bartonella in house rodents in Dehong prefecture of Yunnan province[J]. Disease Surveillance, 2018, 33(1): 20-25. DOI: 10.3784/j.issn.1003-9961.2018.01.006
Citation: Yang Weihong, Song Xiuping, Liang Wei, Feng Yun, Zhang Yuzhen, Zhang Yunzhi, Zhang Hailin, Li Dongmei. Investigation of natural infection status of Bartonella in house rodents in Dehong prefecture of Yunnan province[J]. Disease Surveillance, 2018, 33(1): 20-25. DOI: 10.3784/j.issn.1003-9961.2018.01.006

云南省德宏州居住区啮齿动物携带巴尔通体的调查研究

Investigation of natural infection status of Bartonella in house rodents in Dehong prefecture of Yunnan province

  • 摘要: 目的 调查云南省德宏傣族景颇族自治州(德宏州)居住区啮齿动物的巴尔通体感染和分布特点,为相关疾病防制提供参考依据。方法 采用笼夜法捕鼠,采集鼠类脾脏标本,用胰酶大豆琼脂培养基分离巴尔通体,用聚合酶链式反应法扩增巴尔通体gltA基因片段,并进行核苷酸序列测定和系统发育分析。结果 2011年在德宏州居住区共捕获啮齿动物5属9种340只和食虫动物2种18只,黄胸鼠为优势种(77.09%,276/358)。这些动物的脾脏标本中巴尔通体分离率为24.86%(89/358),其中黄胸鼠分离率为28.99%(80/276),其他阳性鼠种为黑缘齿鼠(5株)、社鼠(1株)、大绒鼠(1株)、小泡灰鼠(1株)和臭鼩鼱(1株)。共获得85株巴尔通体gltA基因核苷酸序列,进化分析表明,40株为特利波契巴尔通体(Bartonella tribocorum),28株为伊丽莎白巴尔通体(B.elizabethae),11株为昆州巴尔通体(B.queenslandensis),3株为森林巴尔通体(B.silvatica),3株未定种。黄胸鼠携带上述所有种类的巴尔通体。结论 德宏州啮齿动物中至少存在4种巴尔通体的流行,巴尔通体具有遗传多样性特点。以特利波契巴尔通体、伊丽莎白巴尔通体和昆州巴尔通体为主要流行菌株,黄胸鼠为当地居住区巴尔通体的主要宿主。今后应加强巴尔通体相关疾病的监测和防制工作。

     

    Abstract: Objective To access the natural infection status of Bartonella in house rodents in Dehong prefecture, Yunnan province, and provide scientific evidence for the prevention and control of Bartonella infection related diseases. Methods Rodents were capture alive by cage traps and their spleen samples were collected to isolate Bartonella by using tryptic soy agar culture media. The gltA gene was amplified and sequenced for constructing the phylogenetic tree. Results A total of 340 rodents in 9 species of 5 categories and 18 insectivorous animals in 2 species were captured from residential area in Dehong in 2011. Rattus flavipectus was the predominant species, accounting for 77.09% (276/358). Bartonella was isolated from the spleen samples of 89 animals (24.86%, 89/358), including 80 Rattus flavipectus (28.99%, 80/276), 5 Rattus andamanensis, 1 Niviventer confucianus, 1 Eothenomys miletus, 1 Berylmys manipulus and 1 Suncus murinus. Based on 85 gltA nucleotide sequences (GenBank No. MF380265-MF380349) from the isolated strains, phylogenetic analysis indicated that 40, 28, 11 and 3 isolates belonged to Bartonella tribocorum, B.elizabethae, B.queenslandensis and B.silvatica, respectively; and 3 isolates could not be identified. R. flavipectus carried all of the Bartonella species mentioned above. Conclusions It is confirmed that at least four Bartonella species were carried by rodents in Dehong, and they had genetic diversity. The main species of Bartonella were B. tribocorum, B.elizabethae and B.queenslandensis. The primary host of Bartonella species was R. flavipectus, which is the most common house rodent in the area. It is necessary to strengthen the surveillance for the diseases related with Bartonella infection in the future.

     

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