Abstract: Objective To analyze the changes of critical indicators of acute flaccid paralysis(AFP)surveillance before and after the application of case tracking management software in Beijing during 2015-2016,and provide scientific evidence for the improvement of the surveillance for AFP. Methods The specificity and timeliness of case investigation,stool sample collection and detection,result feedback,case follow in AFP surveillance in local and floating populations in Beijing during 2015 and 2016 were evaluated. Results A total of 202 and 209 AFP cases were confirmed,including 30 and 44 cases in local population and 172 and 165 cases in floating population,in 2015 and 2016 respectively. The investigation rate within 24 hours after case reporting were all 100% for local cases,while the rate increased from 99.42% to 100% for non-local cases. The double stool sample collection rate within 14 days after paralysis was 96.67% and 93.18%,respectively,for local cases, while it was 72.73% and 65.38%,respectively,for non-local cases. The detection rate of each stool sample within 7 days after collection increased from 93.33% to 100% for local cases and from 70.45% to 96.15% for non-local cases. The result feedback rate within 14 days after sample detection and follow-up rate at 60-70 days after paralysis onset were all 100% for local cases, but they were 98.48% and 97.69%,respectively,for non-local cases. Conclusion The application of AFP case tracking management software greatly facilitated the increase of stool sample detection rate within 7 days after collection in both local and non-local AFP cases in Beijing. However,it had no effect to increase the collection rate of double stool samples within 14 days after paralysis onset.