宋凯丽, 熊衍文, 刁秀念, 郭玉江, 康雁君, 覃新程, 李兴旺. 新疆维吾尔自治区蜱携带白蛉病毒属病毒遗传特征分析[J]. 疾病监测, 2018, 33(8): 670-673. DOI: 10.3784/j.issn.1003-9961.2018.08.014
引用本文: 宋凯丽, 熊衍文, 刁秀念, 郭玉江, 康雁君, 覃新程, 李兴旺. 新疆维吾尔自治区蜱携带白蛉病毒属病毒遗传特征分析[J]. 疾病监测, 2018, 33(8): 670-673. DOI: 10.3784/j.issn.1003-9961.2018.08.014
Kaili Song, Yanwen Xiong, Xiunian Diao, Yujiang Guo, Yanjun Kang, Xincheng Qin, Xingwang Li. Genetic characteristics of phlebovirus carried by ticks in Xinjiang Uygur Autonomous Region[J]. Disease Surveillance, 2018, 33(8): 670-673. DOI: 10.3784/j.issn.1003-9961.2018.08.014
Citation: Kaili Song, Yanwen Xiong, Xiunian Diao, Yujiang Guo, Yanjun Kang, Xincheng Qin, Xingwang Li. Genetic characteristics of phlebovirus carried by ticks in Xinjiang Uygur Autonomous Region[J]. Disease Surveillance, 2018, 33(8): 670-673. DOI: 10.3784/j.issn.1003-9961.2018.08.014

新疆维吾尔自治区蜱携带白蛉病毒属病毒遗传特征分析

Genetic characteristics of phlebovirus carried by ticks in Xinjiang Uygur Autonomous Region

  • 摘要:
    目的 对新疆维吾尔自治区(新疆)蜱携带的白蛉病毒属病毒进行系统进化分析。
    方法 制备蜱悬液提取蜱总RNA,运用反转录聚合酶链式反应筛选阳性标本并扩增白蛉病毒属病毒的全基因组,测序后对病毒基因组进行序列特征和系统发生分析。
    结果 成功扩增3株病毒株(BL10、BL33、BL79)的全长L、S节段。经同源性分析发现,BL10、BL33、BL79与已知蜱携带的Bole Tick Virus 1序列的同源性为98.0% ~ 99.7%;系统进化分析结果表明,BL10、BL33、BL79与Bole Tick Virus 1亲缘关系最近。
    结论 本研究的3株毒株属于白蛉病毒属,且与Bole Tick Virus 1为同种病毒。

     

    Abstract:
    Objective To understand genetic characteristics of Phlebovirus carried by ticks in Xinjiang Uygur Autonomous Region, China.
    Methods Total RNA was extracted from tick homogenate. The screening of phlebovirus positive samples and the amplification of complete genome of phlebovirus in tick samples were performed by using reverse transcriptase polymerase chain reaction, then the genome sequences were used for sequence and phylogenetic analyses.
    Results The complete L and S segment sequences of three strains (BL10, BL33 and BL79) isolated from the tick samples in Xinjiang were amplified successfully. The 3 strains shared 98.0% — 99.7% homology with Bole Tick Virus 1. Additionally, phylogenetic analysis indicated these strains had close relationship with Bole Tick Virus 1.
    Conclusion The three strains belonged to phlebovirus, which could be identified as Bole Tick Virus 1.

     

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