方艳梅, 杨春晓, 张丽荣, 李柏生, 魏泉德. 广东省珠海市两种常见沙门菌血清型的分子分型分析[J]. 疾病监测, 2018, 33(9): 718-723. DOI: 10.3784/j.issn.1003-9961.2018.09.005
引用本文: 方艳梅, 杨春晓, 张丽荣, 李柏生, 魏泉德. 广东省珠海市两种常见沙门菌血清型的分子分型分析[J]. 疾病监测, 2018, 33(9): 718-723. DOI: 10.3784/j.issn.1003-9961.2018.09.005
Yanmei Fang, Chunxiao Yang, Lirong Zhang, Bosheng Li, Quande Wei. Molecular characteristics of two dominant serotypes Salmonella isolated in Zhuhai Guangdong[J]. Disease Surveillance, 2018, 33(9): 718-723. DOI: 10.3784/j.issn.1003-9961.2018.09.005
Citation: Yanmei Fang, Chunxiao Yang, Lirong Zhang, Bosheng Li, Quande Wei. Molecular characteristics of two dominant serotypes Salmonella isolated in Zhuhai Guangdong[J]. Disease Surveillance, 2018, 33(9): 718-723. DOI: 10.3784/j.issn.1003-9961.2018.09.005

广东省珠海市两种常见沙门菌血清型的分子分型分析

Molecular characteristics of two dominant serotypes Salmonella isolated in Zhuhai Guangdong

  • 摘要:
    目的 研究珠海市沙门菌1,4,[5],12:i:–和鼠伤寒沙门菌的耐药和遗传学特征,为食源性疾病分子流行病学调查提供参考数据。
    方法 用纸片法进行抗生素敏感试验,根据PulseNet监测网络公布的沙门菌脉冲场凝胶电泳(PFGE)分型方案与欧洲食源性疾病监测网公布的鼠伤寒沙门菌多位点可变数目串联重复序列分析分型方案,对从腹泻病例分离的121株鼠伤寒沙门菌和S. 1,4,[5],12:i:–进行PFGE和MLVA分型。
    结果 药敏试验结果显示,鼠伤寒沙门菌和S. 1,4,[5],12:i:–的多重耐药株分别为45株(45/51,88.24%)和51株(51/70,72.86%),2种菌多重耐药率差异有统计学意义(χ2=4.256,P=0.039),两者的耐药率>50.00%的抗生素分别是氨苄西林、四环素、复方磺胺甲恶唑和氯霉素。 121株沙门菌经PFGE分型后获得88个型别,2种菌株间没有明显聚类特征,但鼠伤寒沙门菌的型别更为分散,优势型别PT15的11株菌包含了2种血清型。 MLVA分型结果显示5个位点中以STTR5和STTR6的重复数变化最多,分别是11和14个重复数,STTR9和STTR3次之,95.87%的菌株在STTR10p位点无扩增产物。 经MLVA分型后获得50个型别,优势型别为MT18和MT10,分别有23株和11株菌,均包含了2种血清型,经构建最小生成树分析发现91.74%(111株)菌株分布在Ⅰ群。
    结论 珠海市2种菌株的多重耐药率较高,分子分型型别多样,但鼠伤寒沙门菌型别更为分散,两者遗传特征相似。

     

    Abstract:
    Objective To understand the antibiotic resistance and molecular characteristics of Salmonella typhimurium and Salmonella 1,4,[5],12:i:– isolated in Zhuhai, Guangdong province, and provide reference data for molecular epidemiological investigation of food borne diseases.
    Methods Disk diffusion method was used for antibiotic susceptibility test. Pulsed-field gel electrophoresis (PFGE) and multiple loci variable number tandem repeat analysis (MLVA) were conducted for 121 strains of S. typhimurium and S. 1,4,[5],12:i:– isolated from diarrhea patients in Zhuhai by using the protocols published on PulseNet International Network and European Food-borne Disease Surveillance Network.
    Results The antibiotic susceptibility test showed that 45 strains of S. typhimurium (45/51, 88.24%) and 51 strains of S. 1,4,[5],12:i:– (51/70,72.86%) were multidrug resistant, the difference was significant (χ2=4.256, P=0.039). The antibiotics to which two serotypes strains' resistant rates were >50% were ampicillin, tetracycline, compound sulfamethizole and chloramphenicol. PFGE results indicated that the 121 isolates belonged to 88 types. There was no obvious clustering characteristics in two serotypes, but the types of S. typhimurium were more dispersed, the predominant 11 PT15 isolates had two serotypes. MLVA results indicated that STTR5 and STTR6 had highest repeat number (11 and 14), followed by STTR9 and STTR3. Up to 95.87% of the strains had no PCR amplification products. MLVA typing showed that there were 50 types. The predominant types were MT18 and MT10, which contained 23 and 11 isolates respectively, in two serotypes. Analysis with the minimum spanning tree (MST) found that 111 isolates (91.74%) were distributed in group I.
    Conclusion The multidrug resistant rates of two serotypes Salmonella in Zhuhai were high, their molecular types were diverse, but the type of S. typhimurium was more dispersed. They had similar genetic characteristics.

     

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