李颖, 张爽, 王彦波, 张彦春, 张赫, 马红梅. 2014-2017年北京市顺义区腹泻病例中沙门菌流行特征与分子分型特征分析[J]. 疾病监测, 2018, 33(10): 803-808. DOI: 10.3784/j.issn.1003-9961.2018.10.004
引用本文: 李颖, 张爽, 王彦波, 张彦春, 张赫, 马红梅. 2014-2017年北京市顺义区腹泻病例中沙门菌流行特征与分子分型特征分析[J]. 疾病监测, 2018, 33(10): 803-808. DOI: 10.3784/j.issn.1003-9961.2018.10.004
Ying Li, Shuang Zhang, Yanbo Wang, Yanchun Zhang, He Zhang, Hongmei Ma. Epidemiological and molecular characteristics of Salmonella in patients with diarrhea in Shunyi district of Beijing, 2014−2017[J]. Disease Surveillance, 2018, 33(10): 803-808. DOI: 10.3784/j.issn.1003-9961.2018.10.004
Citation: Ying Li, Shuang Zhang, Yanbo Wang, Yanchun Zhang, He Zhang, Hongmei Ma. Epidemiological and molecular characteristics of Salmonella in patients with diarrhea in Shunyi district of Beijing, 2014−2017[J]. Disease Surveillance, 2018, 33(10): 803-808. DOI: 10.3784/j.issn.1003-9961.2018.10.004

2014-2017年北京市顺义区腹泻病例中沙门菌流行特征与分子分型特征分析

Epidemiological and molecular characteristics of Salmonella in patients with diarrhea in Shunyi district of Beijing, 2014−2017

  • 摘要: 目的分析2014 — 2017年北京市顺义区腹泻病例中沙门菌流行特征和分子分型特征,为沙门菌的防控、预警、溯源等提供依据。方法收集北京市顺义区腹泻病例标本和流行病学数据,分离沙门菌并进行脉冲场凝胶电泳(PFGE)分子分型检测,利用国家致病菌识别网平台进行指纹图谱比对,结合实验室检测结果、流行病学资料、临床资料对本地区沙门菌流行和分子分型特征进行描述分析。结果顺义区腹泻病例中沙门菌检出率为5.23%,21种血清型,以鼠伤寒沙门菌(32.89%)和肠炎沙门菌(30.26%)为主;沙门菌检出率呈春秋季双高峰分布;女性和男性肠炎沙门菌的检出率差异有统计学意义(χ2=5.978,P=0.014);发热体温越高的病例组,沙门菌属检出率越高,差异有统计学意义(χ2=22.579,P<0.001);菌株PFGE分析结果显示存在多次聚集的现象。结论沙门菌在北京市顺义区腹泻病原中占有重要位置;不同血清型沙门菌的人群分布、临床症状分布和分子分型分布均具有一定特征;利用国家致病菌识别网识别、预警、评估、溯源沙门菌引起的食源性疾病和肠道传染病是本地区沙门菌防控工作的方向。

     

    Abstract: ObjectiveTo understand epidemiological and molecular characteristics of Salmonella in patients with diarrhea in Shunyi district of Beijing and provide evidence for the prevention and control of Salmonellainfection.MethodsThe stool samples of diarrhea patients were collected for Salmonella isolation and the molecular typing of the isolates was performed with pulsed field gel electrophoresis (PFGE). Statistical, comparative and descriptive analyses were conducted by suing the epidemiological data of the diarrhea patients.ResultsThe detection rate of Salmonella was 5.23% in the diarrhea patients in Shunyi. Twenty one serotypes were detected, S. Typhimurium (32.89%) and S.Enteritis (30.26%) were predominant. The peaks of Salmonella detection occurred in Spring and Autumn; There was a significant difference in detection rate of S. Enteritidis between males and females; The patients in higher body temperature group had higher detection rate of Salmonella, the difference was significant (χ2=22.579, P<0.001). The PFGE analysis showed multiple aggregation phenomenon.ConclusionSalmonella is a major diarrhea pathogen in Shunyi district of Beijing. Salmonella with different serotypes showed different epidemiological and molecular characteristics. It is necessary to conduct identification, early-warning, tracing and assessment for Salmonella caused foodborne diseases and enteric infectious diseases by using China Pathogenic Identification Net.

     

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