陶霞, 张晶, 魏跃红, 周勇, 白志军, 吴新伟. 2012-2016年广州市人感染恙虫病东方体基因型与流行病学特征分析[J]. 疾病监测, 2018, 33(11): 902-907. DOI: 10.3784/j.issn.1003-9961.2018.11.006
引用本文: 陶霞, 张晶, 魏跃红, 周勇, 白志军, 吴新伟. 2012-2016年广州市人感染恙虫病东方体基因型与流行病学特征分析[J]. 疾病监测, 2018, 33(11): 902-907. DOI: 10.3784/j.issn.1003-9961.2018.11.006
Xia Tao, Jing Zhang, Yuehong Wei, Yong Zhou, Zhijun Bai, Xinwei Wu. Genotypic and epidemiological analysis of Orientale tsutsugamushi infection in Guangzhou, 2012–2016[J]. Disease Surveillance, 2018, 33(11): 902-907. DOI: 10.3784/j.issn.1003-9961.2018.11.006
Citation: Xia Tao, Jing Zhang, Yuehong Wei, Yong Zhou, Zhijun Bai, Xinwei Wu. Genotypic and epidemiological analysis of Orientale tsutsugamushi infection in Guangzhou, 2012–2016[J]. Disease Surveillance, 2018, 33(11): 902-907. DOI: 10.3784/j.issn.1003-9961.2018.11.006

2012-2016年广州市人感染恙虫病东方体基因型与流行病学特征分析

Genotypic and epidemiological analysis of Orientale tsutsugamushi infection in Guangzhou, 2012–2016

  • 摘要:
    目的 了解广州市2012 — 2016年人感染恙虫病东方体的基因分型与流行病学特征。
    方法 收集2012 — 2016年广州市临床诊断恙虫病患者全血,利用巢式聚合酶链式反应(PCR)扩增56 kDa型特异性抗原基因并测序,通过MEGA 5.0软件构建系统发生树并进行同源性分析。
    结果 共收集到临床诊断恙虫病患者标本906份,其中220份经巢式PCR检测阳性(阳性率24.3%),包括Karp型138株、Kato型29株、Gilliam型39株和TA763型13株,另有1株未确定型别。 两个发病高峰期为5 — 6月和9 — 10月,发病区域集中在从化区、增城区等农村地区。
    结论 近年来广州市恙虫病东方体基因型呈现多样性,但以Karp型为主要流行型,需要加强对恙虫病东方体的分子流行病学监测分析工作。

     

    Abstract:
    Objective To analyze the genotypic and epidemiological characteristics of Orientia tsutsugamushi infection in Guangzhou from 2012 to 2016.
    Methods Whole blood samples were collected from the clinical diagnosed patients. 56kDa type-specific antigen gene segment of O. tsutsugamushi was amplified by using nested-polymerase chain reaction. Sequences were analyzed by alignment with GenBank and the phylogenetic tree was established by using software MGEA 5.0.
    Results A total of 220 samples were detected to be positive (24.3%) from 906 samples, including 138 strains of Karp, 29 strains of Kato, 39 strains of Gilliam, 13 strains of TA763 and 1 strains of uncertain genotype. Two incidence peaks were observed, one was during May - June and another one was during September - October. The cases mainly occurred in rural areas of Conghua and Zengcheng.
    Conclusion The incidence of scrub typhus in Guangzhou was in increase and the genotypes of O. tsutsugamushi showed diversity, but Karp was predominant. It is necessary to strengthen the molecular epidemiology surveillance for O. tsutsugamushi in Guangzhou.

     

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