岳峰, 方正, 孙永, 崔志刚. 2013-2015年安徽省合肥市食源性疾病患者分离沙门菌的病原学特征分析[J]. 疾病监测, 2019, 34(4): 312-315. DOI: 10.3784/j.issn.1003-9961.2019.04.008
引用本文: 岳峰, 方正, 孙永, 崔志刚. 2013-2015年安徽省合肥市食源性疾病患者分离沙门菌的病原学特征分析[J]. 疾病监测, 2019, 34(4): 312-315. DOI: 10.3784/j.issn.1003-9961.2019.04.008
Feng Yue, Zheng Fang, Yong Sun, Zhigang Cui. Etiological characteristics of clinical Salmonella isolates from foodborne infection patients in Hefei, 2013–2015[J]. Disease Surveillance, 2019, 34(4): 312-315. DOI: 10.3784/j.issn.1003-9961.2019.04.008
Citation: Feng Yue, Zheng Fang, Yong Sun, Zhigang Cui. Etiological characteristics of clinical Salmonella isolates from foodborne infection patients in Hefei, 2013–2015[J]. Disease Surveillance, 2019, 34(4): 312-315. DOI: 10.3784/j.issn.1003-9961.2019.04.008

2013-2015年安徽省合肥市食源性疾病患者分离沙门菌的病原学特征分析

Etiological characteristics of clinical Salmonella isolates from foodborne infection patients in Hefei, 2013–2015

  • 摘要:
    目的分析安徽省合肥市食源性疾病患者所分离沙门菌的血清型别以及脉冲场凝胶电泳(PFGE)分子型别特点。
    方法对2013 — 2015年在合肥市2家哨点医院就诊的2 618例食源性疾病患者,进行沙门菌的分离鉴定以及血清学分析,并用Xba Ⅰ酶切进行PFGE分析其分子型别特征。
    结果在2 618份患者标本中,共检测出76株沙门菌,总检出率为2.9%,共分为17种血清型,鼠伤寒沙门菌检出率最高,其次为肠炎沙门菌。 69株沙门菌经Xba Ⅰ酶切,共产生53种PFGE带型,每种带型包含1 ~ 5株沙门菌,其中鼠伤寒沙门菌共产生20种带型,肠炎沙门菌共产生10种带型。
    结论初步了解合肥市食源性疾病沙门菌感染是一种以鼠伤寒沙门菌和肠炎沙门菌为主、多血清型长期共存的散发性感染,获得了本地区食源性疾病沙门菌感染的基线水平的血清型别和PFGE分子分型数据库,为后续暴发监测奠定了基础。

     

    Abstract:
    ObjectiveTo study the serotypes and molecular characteristics of Salmonella isolates from the foodborne infection patients in Hefei of Anhui province.
    MethodsThe isolation and identification of Salmonella were conducted by using the clinical samples collected from the foodborne infection patients in 2 sentinel hospitals in Hefei during 2013 and 2015, the serotypes and pulse field gel electrophoresis (PFGE) patterns by Xba Ⅰ enzymes digestion of the Salmonella isolates were analyzed.
    ResultsA total of 76 Salmonella strains were isolated from 2 618 clinical samples, the overall isolation rate was 2.9%. Among the 76 Salmonella isolates, 17 serotypes were detected. The major serotype was S. typhimurium, followed by S. enteriditics. Fifty three molecular patterns were found with Xba Ⅰ enzymes digestion in 69 Salmonella strains, each pattern contained 1-5 strains of Salmonella. Among them, S. typhimurium had 20 molecular patterns, and S. enteriditics had 10 molecular patterns.
    ConclusionFoodborne Salmonella infection was mainly caused by S. typhimurium and S. enteriditics in Hefe with characteristics of co-existing of multi serotypes and sporadic outbreak. The establishment of serotype and PFGE molecular pattern databases of Salmonella causing food borne infection would facilitate the surveillance for foodborne Salmonella infection outbreak.

     

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