袁月, 钟佑宏, 张海鹏, 苏超, 杨丽华, 杨丰义, 吴鹤松, 王鹏. 云南省褐家鼠中鼠疫噬菌体的分离及其流行病学意义[J]. 疾病监测, 2020, 35(3): 227-230. DOI: 10.3784/j.issn.1003-9961.2020.03.011
引用本文: 袁月, 钟佑宏, 张海鹏, 苏超, 杨丽华, 杨丰义, 吴鹤松, 王鹏. 云南省褐家鼠中鼠疫噬菌体的分离及其流行病学意义[J]. 疾病监测, 2020, 35(3): 227-230. DOI: 10.3784/j.issn.1003-9961.2020.03.011
Yue Yuan, Youhong Zhong, Haipeng Zhang, Chao Su, Lihua Yang, Fengyi Yang, Hesong Wu, Peng Wang. Isolation of Yersinia pestis phage from Rattus norvegicus in Yunnan province and its epidemiological significance[J]. Disease Surveillance, 2020, 35(3): 227-230. DOI: 10.3784/j.issn.1003-9961.2020.03.011
Citation: Yue Yuan, Youhong Zhong, Haipeng Zhang, Chao Su, Lihua Yang, Fengyi Yang, Hesong Wu, Peng Wang. Isolation of Yersinia pestis phage from Rattus norvegicus in Yunnan province and its epidemiological significance[J]. Disease Surveillance, 2020, 35(3): 227-230. DOI: 10.3784/j.issn.1003-9961.2020.03.011

云南省褐家鼠中鼠疫噬菌体的分离及其流行病学意义

Isolation of Yersinia pestis phage from Rattus norvegicus in Yunnan province and its epidemiological significance

  • 摘要:
    目的调查云南省鼠疫宿主动物褐家鼠中携带鼠疫噬菌体的情况,并探讨其流行病学意义。
    方法2015 — 2018年在云南省10个县(市)25个点进行采样,取捕获的褐家鼠肠道标本,以鼠疫疫苗株EV76为饲养菌,采用双层平板法分离肠道标本中的鼠疫噬菌体;同时对肠道标本进行鼠疫菌特异基因caf1检测。
    结果共采集344份褐家鼠标本,从中分离到15株鼠疫噬菌体,总分离率为4.36%;从采样点看,10个点中分离到鼠疫噬菌体,玉溪市红塔区的春和街及玉带街分离率最高(分别为28.57%和16.67%);不同性别、年龄和生境鼠疫噬菌体的分离率差异无统计学意义;所分离的鼠疫噬菌体中有4株为肌尾型;所有标本caf1检测阴性。
    结论云南省褐家鼠中存在一定数量的鼠疫噬菌体,且鼠疫历史疫区、近史流行区、复燃疫区及现疫区中均有分布;历史疫区及城区褐家鼠中鼠疫噬菌体携带率较高,其微生态意义值得进一步探讨。

     

    Abstract:
    ObjectiveTo investigate the status of plague phages in Rattus norvegicus, the host animal of plague in Yunnan province, and to explore its epidemiological significance.
    MethodsFrom 2015 to 2018, samples were taken from 25 sites in 10 counties and cities of Yunnan province to collect intestinal specimens of captured Rattus norvegicus. The Yersinia pestis phage was isolated from intestinal specimens by double plate method. The specific gene caf1 of Yersinia pestis was detected.
    ResultsTotally 344 copies of brown mouse were collected and 15 strains of Yersinia pestis phages were isolated from them, with a total isolation rate of 4.36%. From the sampling points, 10 spots were isolated from Yersinia pestis phages, with the highest isolation rates of Chunhe street and Yudai street in Hongta district of Yuxi city (28.57% and 16.67%). There was no significant difference between the sex, age and habitat of rats on the isolation of Yersinia pestis phages.The caf1 test of all specimens was negative. Four of the isolated bacteriophages are of the Myoviridae family.
    ConclusionThere are a certain number of Yersinia pestis phages in Rattus norvegicus in Yunnan province, and they are distributed in the historical epidemic area, the near historical epidemic area, the rekindling epidemic area and the current epidemic area. The carrying rate of Yersinia pestis phages in the historical epidemic area and urban Rattus norvegicus is higher, which microecological significance is worth further discussion.

     

/

返回文章
返回