胃黏膜相关淋巴组织淋巴瘤幽门螺杆菌分离株与胃炎分离株的转录组比较

王磊; 邹清华; 张慧芳; 张建中; 肖迪

doi:10.3784/j.issn.1003-9961.2020.04.005

胃黏膜相关淋巴组织淋巴瘤幽门螺杆菌分离株与胃炎分离株的转录组比较

Comparisons of transcriptome between gastric MALT isolates and gastric ulcer isolates of Helicobacter pylori

摘要

摘要:
目的本研究对胃黏膜相关淋巴组织淋巴瘤（MALT淋巴瘤）幽门螺杆菌分离株与胃炎分离株的转录组进行比较，以阐述胃MALT淋巴瘤幽门螺杆菌分离株的转录组特征。
方法对2株分离自胃MALT淋巴瘤的菌株与胃炎菌株26695的转录组进行比较。
结果胃MALT淋巴瘤幽门螺杆菌分离株之间转录组非常相似，而与胃炎菌株26695之间差异较大。对差异表达基因进行GO功能富集分析，结果显示富集基因数前5位的为催化活性、代谢进程、细胞进程、结合、单组织进程。 Pathway显著性富集分析表明差异基因的功能主要参与氨基酸和核苷酸糖代谢、卟啉和叶绿素代谢以及乙醛和二羧酸代谢。
结论胃MALT淋巴瘤幽门螺杆菌相关菌株可能通过代谢调控菌体生长和侵染宿主，从而在胃MALT淋巴瘤形成过程中发挥着重要作用。

Abstract:
ObjectiveHelicobacter pylori is the main cause of gastric mucosa-associated lymphoid tissue lymphoma (MALT lymphoma). This study aimed to characterize the transcriptome characteristics of gastric MALT lymphoma isolates in comparison with gastritis isolate of H. pylori.
MethodsThe transcriptome of two gastric MALT lymphoma isolates and gastritis isolate (26695) of H. pylori were compared.
ResultsThe transcriptome of the two MALT lymphoma isolates were very similar, in contrast, there was great difference between the MALT lymphoma isolates and gastritis isolate (26695). GO enrichment analysis showed that the top five enriched items were catalytic activity, binding, metabolic process, cellular process, and single-organism process. Pathway enrichment showed that the differentially transcribed genes were mainly with functions in amino sugar and nucleotide sugar metabolism, porphyrin and chlorophyll metabolism, and glyoxylate and dicarboxylate metabolism.
ConclusionH. pylori gastric MALT lymphoma isolates may play an important role in the formation of gastric MALT lymphoma by metabolic regulation of bacterial growth and infection of host.

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