黄邵军, 罗学辉, 诸佳辉. 2018-2019年浙江省余姚市河水环境中沙门菌多样性及耐药性分析[J]. 疾病监测, 2020, 35(10): 946-952. DOI: 10.3784/j.issn.1003-9961.2020.10.016
引用本文: 黄邵军, 罗学辉, 诸佳辉. 2018-2019年浙江省余姚市河水环境中沙门菌多样性及耐药性分析[J]. 疾病监测, 2020, 35(10): 946-952. DOI: 10.3784/j.issn.1003-9961.2020.10.016
Shaojun Huang, Xuehui Luo, Jiahui Zhu. Diversity and drug resistance of Salmonella in river environment of Yuyao, Zhejiang, 2018–2019[J]. Disease Surveillance, 2020, 35(10): 946-952. DOI: 10.3784/j.issn.1003-9961.2020.10.016
Citation: Shaojun Huang, Xuehui Luo, Jiahui Zhu. Diversity and drug resistance of Salmonella in river environment of Yuyao, Zhejiang, 2018–2019[J]. Disease Surveillance, 2020, 35(10): 946-952. DOI: 10.3784/j.issn.1003-9961.2020.10.016

2018-2019年浙江省余姚市河水环境中沙门菌多样性及耐药性分析

Diversity and drug resistance of Salmonella in river environment of Yuyao, Zhejiang, 2018–2019

  • 摘要:
    目的了解浙江省余姚地区河水中沙门菌的分布和污染情况。
    方法2018 — 2019年采集余姚市12个监测点的河水,进行增菌、分离、生化鉴定、血清分型、药敏试验及耐药基因检测。
    结果采集河水样品192份,检出含有沙门菌样品116份,检出率为60.42%,其中姚江姚江桥点检出率最高(87.50%)。 阳性水样共检出菌株177株,分为25个血清型,其中鼠伤寒沙门菌(16.38%)、德尔卑沙门菌(12.43%)和鼠伤寒单相变种(11.30%)位居前3位,各监测点中以姚江三管堂点血清型检出最多。 春季分离到的血清型别更丰富。 39株表现为对所测试的16种抗生素泛敏感,而其他菌株则表现为至少对1种抗生素表现出耐药性。 耐药率较高的为青霉素类和四环素类抗生素,其中氨苄西林耐药率为72.31%,哌拉西林为51.41%,四环素为66.10%。 对3种及以上的抗生素耐药的多重耐药菌菌株有34株。 β-内酰胺类抗生素相关耐药基因tem检出率为67.23%,oxa为6.78%,ctx-M为12.43%;四环素类抗生素相关耐药基因则分别为tetA型(55.37%)、tetB型(11.30%)和tetG型(7.91%)。
    结论余姚地区河水中沙门菌检出率高、菌型丰富、耐药率严重、耐药机制复杂,食源性防控中应注意环境来源的沙门菌的危害。

     

    Abstract:
    ObjectiveTo understand the distribution and pollution of Salmonella in rivers in Yuyao, Zhejaing province.
    MethodsThe water samples were collected from 12 surveillance sites in Yuyao from January 2018 to December 2019, and then enrichment, isolation, biochemical identification, serotyping, drug sensitivity test and detection of drug resistance genes were carried out.
    ResultsA total of 116 water samples containing Salmonella were detected in 192 river water samples, with a detection rate of 60.42%. The positive rate at Yaojiang bridge was highest (87.50%). A total of 177 Salmonella strains were detected in 116 positive water samples, which belonged to 25 serotypes, of which S.Typhimurium (16.38%), S. Derby (12.43%) and S.4,[5],12:i:- (11.30%) ranked top three. The number of serotypes detected at Sanguantang of Yaojiang river was highest. In seasonal distribution of the detection, more serotypes were detected in spring. Thirty nine strains showed extensive sensitivity to 16 antibiotics tested, while other strains showed resistance to at least one antibiotic. The resistance rates to penicillin and tetracycline were higher, the resistance rates to ampicillin, piperacillin and tetracycline were 72.31%, 51.41% and 66.10%, respectively. Thirty four strains were multi-drug resistant to three or more antibiotics. The detection rates of β-lactam antibiotic resistance gene tem, oxa and ctx-M were 67.23%, 6.78% and 12.43% respectively. The detection rates of tetracycline resistance gene tetA, tetB and tetG were 55.37%, 11.30% and 7.91%, respectively.
    ConclusionThe detection rate of Salmonella in river environment of Yuyao was high, and the detected Salmonella strains had multi serotypes, serious drug resistance and complicated drug resistance mechanism. Therefore, it is suggested to pay close attention to the hazard of Salmonella from environmental sources in foodborne disease control.

     

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