贾琴妹, 杨传莲, 黄艳梅. VITEK-2 Compact全自动微生物鉴定仪与VITEK MS质谱仪在摩根摩根菌鉴定中的应用及评价[J]. 疾病监测, 2020, 35(11): 1025-1030. DOI: 10.3784/j.issn.1003-9961.2020.11.014
引用本文: 贾琴妹, 杨传莲, 黄艳梅. VITEK-2 Compact全自动微生物鉴定仪与VITEK MS质谱仪在摩根摩根菌鉴定中的应用及评价[J]. 疾病监测, 2020, 35(11): 1025-1030. DOI: 10.3784/j.issn.1003-9961.2020.11.014
Jia Qinmei, Yang Chuanlian, Huang Yanmei. Application and evaluation of VITEK-2 Compact and VITEK MS in identification of Morganella morganii[J]. Disease Surveillance, 2020, 35(11): 1025-1030. DOI: 10.3784/j.issn.1003-9961.2020.11.014
Citation: Jia Qinmei, Yang Chuanlian, Huang Yanmei. Application and evaluation of VITEK-2 Compact and VITEK MS in identification of Morganella morganii[J]. Disease Surveillance, 2020, 35(11): 1025-1030. DOI: 10.3784/j.issn.1003-9961.2020.11.014

VITEK-2 Compact全自动微生物鉴定仪与VITEK MS质谱仪在摩根摩根菌鉴定中的应用及评价

Application and evaluation of VITEK-2 Compact and VITEK MS in identification of Morganella morganii

  • 摘要:
      目的  分析研究VITEK-2 Compact全自动微生物鉴定仪与VITEK MS质谱仪在摩根摩根菌鉴定中的应用及对其鉴定结果的准确性做出评价,为临床精准治疗提供科学依据。
      方法  收集2017年1月至2020年5月云南省第三人民医院送检的不同类型标本中分离出的50株摩根摩根菌;常规方法进行菌株培养;采用VITEK-2 Compact全自动微生物鉴定仪,手工生化补充试验和VITEK MS质谱仪对菌株进行菌种鉴定,鉴定结果不一致的菌株采用16S rDNA测序验证。
      结果  共分离出5株β溶血表型和45株非β溶血表型摩根摩根菌;VITEK-2 Compact全自动微生物鉴定仪对非β溶血摩根摩根菌鉴定结果均符合,对β溶血摩根摩根菌鉴定结果均不符合,且均鉴定为奇异变形杆菌,鉴定符合率为90%;手工生化补充试验结果也符合奇异变形杆菌;VITEK MS质谱仪对非β溶血和β溶血摩根摩根菌的鉴定结果均符合,鉴定符合率为100%;16S rDNA测序对鉴定结果不一致的5株β溶血菌的测序结果均为摩根摩根菌。
      结论  VITEK-2 Compact全自动微生物鉴定仪对非β溶血摩根摩根菌的鉴定结果准确可信;对β溶血摩根摩根菌鉴定结果均不准确,且鉴定为奇异变形杆菌的概率很高;VITEK MS质谱仪对摩根摩根菌的鉴定结果均准确快速且不受细菌β溶血与否影响。

     

    Abstract:
      Objective  To analyze the application of VITEK-2 Compact and VITEK MS in the identification of Morganella morganii, evaluate the accuracy of the identification results and provides evidence for clinical precision treatment.
      Methods  We collected 50 strains of M. morganii isolated from different types of samples in our hospital from January 2017 to May 2020. The bacterial strains were cultured by conventional methods. Then, VITEK-2 Compact, manual biochemical supplementary test and VITEK MS were used to identify the strains. The strains with different identification results were verified by 16S rDNA sequencing.
      Results  The 50 strains of M. morganii were composed of 5 strains of beta-hemolytic phenotype and 45 strains of non-beta-hemolytic phenotype. VITEK-2 Compact had consistent identification results for non-beta-hemolytic M. morganii, but not for beta-hemolytic M. morganii, which were all identified as Proteus mirabilis, the identification consistent rate was 90%, and manual biochemical supplementary test had same results. The identification results of VITEK MS for non-beta-hemolytic and beta-hemolytic M. morganii were consistent, and the identification consistent rates were 100%. 16S rDNA sequencing verified the five beta-hemolytic strains of M. morganii, which had non-consistent identification results in previous identifications.
      Conclusion  VITEK-2 Compact was accurate and reliable for the identification of non-beta hemolytic M. morganii, but its identifications of beta-hemolytic M. morganii were inaccurate, and the probability of false identification as Proteus mirabilis was very high. The identification result of VITEK MS for M. morganii was accurate and it was not affected by the beta-hemolytic phenotype.

     

/

返回文章
返回