李静, 张婷, 邹文菁, 蔡昆, 徐军强. 2018-2019年湖北省5岁及以下腹泻儿童诺如病毒感染特征和病原学分析[J]. 疾病监测, 2021, 36(4): 369-375. DOI: 10.3784/jbjc.202101050006
引用本文: 李静, 张婷, 邹文菁, 蔡昆, 徐军强. 2018-2019年湖北省5岁及以下腹泻儿童诺如病毒感染特征和病原学分析[J]. 疾病监测, 2021, 36(4): 369-375. DOI: 10.3784/jbjc.202101050006
Li Jing, Zhang Ting, Zou Wenjing, Cai Kun, Xu Junqiang. Epidemiological and etiological characteristics of norovirus infection in children under 5 years old in Hubei, 2018–2019[J]. Disease Surveillance, 2021, 36(4): 369-375. DOI: 10.3784/jbjc.202101050006
Citation: Li Jing, Zhang Ting, Zou Wenjing, Cai Kun, Xu Junqiang. Epidemiological and etiological characteristics of norovirus infection in children under 5 years old in Hubei, 2018–2019[J]. Disease Surveillance, 2021, 36(4): 369-375. DOI: 10.3784/jbjc.202101050006

2018-2019年湖北省5岁及以下腹泻儿童诺如病毒感染特征和病原学分析

Epidemiological and etiological characteristics of norovirus infection in children under 5 years old in Hubei, 2018–2019

  • 摘要:
      目的  了解2018 — 2019年湖北省≤5岁腹泻儿童诺如病毒感染流行病学及分子特征。
      方法  收集2018 — 2019年湖北省病毒性腹泻监测哨点医院≤5岁腹泻儿童粪便标本,共计922份。 采用实时荧光反转录聚合酶链式反应(real-time RT-PCR)方法进行诺如病毒GⅠ和GⅡ组核酸检测。 对诺如病毒检测阳性的标本应用real-time RT-PCR分别扩增RNA聚合酶和衣壳蛋白片段,将获得的PCR产物直接测序并进行基因型别分析。
      结果  湖北省≤5岁腹泻患儿粪便标本中,诺如病毒核酸阳性样本为122份,阳性率13.23%。其中诺如病毒GⅡ组119例(97.54%),诺如病毒GⅠ组3例(2.46%)。 男女童感染者差异有统计学意义(χ2=8.805,P=0.003)。 ≤5岁儿童以6~12月龄为主要发病人群。 诺如病毒感染季节为冬春季,呈现2个流行峰,10 — 12月为高流行峰;诺如病毒主要为GⅡ组,聚合酶区和衣壳区基因分型主要为GⅡ.4 Sydney[P31](27.71%)、GⅡ.2[P16](26.51%)、GⅡ.3[P12](26.51%)和GⅡ.4 Sydney[P16](16.87%)。
      结论  2018 — 2019年湖北地区≤5岁腹泻患儿感染诺如病毒存在多种基因型和不同的重组亚型,加强诺如病毒的基因型别监测和重点人群防护有助于诺如病毒腹泻感染的防控。

     

    Abstract:
      Objective  To understand the epidemiological and molecular characteristics of norovirus associated with acute diarrhea in infants and young children in Hubei province from 2018 to 2019.
      Methods  A total of 922 stool samples from children aged ≤5 years with diarrhea were collected from viral diarrhea surveillance sentinel hospitals in Hubei Province from 2018 to 2019. The nucleic acids of norovirus GⅠ and GⅡ were detected by reverse transcription polymerase chain reactions. RT-PCR was used to amplify RNA polymerase and capsid region of the strain from norovirus positive samples and the PCR products were sequenced and genotyped.
      Results  Among the 922 stool samples collected, 122 were norovirus positive (13.23%), in which 119 were norovirus GⅡ positive (97.54%) and 3 were norovirus GⅠ positive (2.46%). The difference in the infection rate between boys and girls was significant ( χ2=8.805, P=0.003). Children aged ≤5 years, especially the infants aged6 - 12 months, were the population with high incidence. Norovirus infection mainly occurred in winter and spring, there were two incidence peaks, and the higher one occurred during October-December. Norovirus GⅡ was predominant and recombination genotypes were mainly GⅡ.4 Sydney [P31](27.71%), GⅡ.2[P16](26.51%), GⅡ.3[P12](26.51%) and GⅡ.4 Sydney [P16](16.87%).
      Conclusion  Multi genotypes and different recombination subtypes of norovirus were detected in children aged≤5 years with diarrhea in Hubei from 2018 to 2019. It is necessary to strengthen the surveillance for norovirus genotype and protect key population for the prevention and control of norovirus infection.

     

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