张浩然, 黄勇, 梁蓓蓓, 杜昕颖, 邱少富, 宋宏彬, 向莹, 袁正泉, 李劲松. 一株mcr-1阳性韦太夫雷登沙门菌的鉴定和药物敏感性特征分析[J]. 疾病监测, 2021, 36(6): 628-633. DOI: 10.3784/jbjc.202102150067
引用本文: 张浩然, 黄勇, 梁蓓蓓, 杜昕颖, 邱少富, 宋宏彬, 向莹, 袁正泉, 李劲松. 一株mcr-1阳性韦太夫雷登沙门菌的鉴定和药物敏感性特征分析[J]. 疾病监测, 2021, 36(6): 628-633. DOI: 10.3784/jbjc.202102150067
Zhang Haoran, Huang Yong, Liang Beibei, Du Xinying, Qiu Shaofu, Song Hongbin, Xiang Ying, Yuan Zhengquan, Li Jinsong. Identification and antimicrobial resistance analysis of mcr-1 positive Salmonella Weltevreden[J]. Disease Surveillance, 2021, 36(6): 628-633. DOI: 10.3784/jbjc.202102150067
Citation: Zhang Haoran, Huang Yong, Liang Beibei, Du Xinying, Qiu Shaofu, Song Hongbin, Xiang Ying, Yuan Zhengquan, Li Jinsong. Identification and antimicrobial resistance analysis of mcr-1 positive Salmonella Weltevreden[J]. Disease Surveillance, 2021, 36(6): 628-633. DOI: 10.3784/jbjc.202102150067

一株mcr-1阳性韦太夫雷登沙门菌的鉴定和药物敏感性特征分析

Identification and antimicrobial resistance analysis of mcr-1 positive Salmonella Weltevreden

  • 摘要:
      目的  鉴定1 株 mcr-1 阳性韦太夫雷登沙门菌并对其耐药特征及机制进行分析。
      方法  基于肠道病原监测平台收集的分离于2015年的沙门菌株使用生化检测试验和血清凝集试验进行病原体鉴定和血清型分型,并用聚合酶链式反应方法检测mcr-1基因;使用微量肉汤稀释法进行药敏试验;使用质粒图谱和Southern-blot试验对菌株耐药基因mcr-1进行定位;用质粒接合转移试验验证mcr-1质粒的水平转移能力;提取细菌的质粒进行质粒测序,并对质粒序列进行注释分析及图谱的绘制。
      结果  鉴定出1株mcr-1阳性的韦太夫雷登沙门菌,该菌株对多粘菌素耐药。 质粒图谱和Southern-blot结果显示mcr-1基因定位在约30 kb质粒上,质粒接合转移实验结果显示该质粒可以在肠道菌株之间水平转移。 质粒测序分析结果表明携带mcr-1基因的质粒pS68大小为32914 bp,类型为IncX4,该质粒同时携带有5个毒力基因。
      结论  加强食源性病原菌监测和mcr-1耐药基因转移的分子流行病学研究,为更好的遏制耐药菌株的流行扩散提供坚实的基础。

     

    Abstract:
      Objective  To identify mcr-1 positive Salmonella and analyze its antibiotic resistance characteristics and mechanism.
      Methods  Pathogen biochemical identification and serotyping with serological agglutination test were conducted for the Salmonella strains isolated in 2015 through intestinal tract pathogen surveillance platform, the mcr-1 gene was detected with PCR and microbroth dilution method was used for drug susceptibility testing of the strains. S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) and southern-blot analysis was performed to locate the resistance gene mcr-1. To verify the horizontal transfer ability of mcr-1 plasmids, plasmid conjugative transfer assay was used. The strains were sequenced by the miseq platform sequencer of Illumina, and the assembled plasmid sequences were annotated and analyzed.
      Results  A mcr-1-positive Salmonella Weltevreden was identified, which was resistant to polymyxin. S1-PFGE and Southern-blot revealed that mcr-1 gene was located on a plasmid which was about 30 kb size. The result of plasmid conjugative transfer assay showed that the plasmid could be transferred horizontally. The plasmid sequencing analysis indicated that the plasmid, named as pS68, had a size of 32914 bp, belonged to IncX4, carried five virulence genes.
      Conclusion  We should strengthen the surveillance of foodborne pathogens and the molecular epidemiology research of mcr-1 resistance gene transfer, so as to lay a sound foundation for better prevention of spread of drug resistant strains.

     

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