类鼻疽伯克霍尔德菌在青瓜和生菜幼苗中的定植情况研究

陈少金; 王立程; 朱雄; 符晓莹; 陈海; 梁丽娟; 韩丽梅

doi:10.3784/jbjc.202103160128

类鼻疽伯克霍尔德菌在青瓜和生菜幼苗中的定植情况研究

Colonization of Burkholderia pseudomallei in cucumber and lettuce seedlings

摘要

摘要:
目的研究类鼻疽伯克霍尔德菌在青瓜、生菜幼苗中的定植情况。
方法用海南省三亚市人民医院保存的类鼻疽伯克霍尔德菌BP47、BP56、BP66分别感染青瓜、生菜无菌幼苗根部和叶片，从被感染幼苗根部与叶片的研磨液中分离该菌。采用菌落平板计数检测被感染第1、3、5、7天的幼苗叶片中类鼻疽伯克霍尔德菌的增殖情况，绘制增殖曲线。通过透射电镜对幼苗叶片中的类鼻疽伯克霍尔德菌进行定位。
结果从被感染的青瓜、生菜幼苗中分离到了类鼻疽伯克霍尔德菌，而且该菌可以在幼苗中增殖，菌量随着时间推移而增加。通过透射电镜在青瓜、生菜幼苗叶片中也观察到了该菌定植。
结论类鼻疽伯克霍尔德菌可以在一些生食类蔬菜（青瓜、生菜）幼苗中定植，为探究其新的感染方式提供了实验依据。

Abstract:
Objective To study the colonization of Burkholderia pseudomallei in cucumber and lettuce seedlings.
Methods Germ-free seedlings of cucumber and lettuce were infected by B. pseudomallei BP56, BP66, BP47, which were preserved at Sanya People′s Hospital in Hainan province, via roots and leaves. The grinding fluid of infected roots and leaves were used for isolation of B. pseudomallei. The proliferation of B. pseudomallei were measured from the seedling leaves on day 1, 3, 5 and 7 after infection by plate counting method. And the curves of B. pseudomallei proliferation were plotted. B. pseudomallei in seedling leaves were located by transmission electron microscopy.
Results B. pseudomallei were isolated from infected cucumber and lettuce seedlings, and B. pseudomallei proliferated in seedlings over time following the infection. The colonization of B. pseudomallei in the leaves of cucumber and lettuce seedlings was observed by transmission electron microscopy.
Conclusion B. pseudomallei can replicate in some raw vegetables, such as cucumber, lettuce. It is the new laboratory evidences to study the possible infection mode of B. pseudomallei.

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