樊玲芳, 张京云, 沙丹, 管红霞. FilmArray G Panel在腹泻病原体检测中的应用[J]. 疾病监测, 2022, 37(6): 807-812. DOI: 10.3784/jbjc.202104070187
引用本文: 樊玲芳, 张京云, 沙丹, 管红霞. FilmArray G Panel在腹泻病原体检测中的应用[J]. 疾病监测, 2022, 37(6): 807-812. DOI: 10.3784/jbjc.202104070187
Fan Lingfang, Zhang Jingyun, Sha Dan, Guan Hongxia. Application of FilmArray G Panel in the detection of diarrhea pathogens[J]. Disease Surveillance, 2022, 37(6): 807-812. DOI: 10.3784/jbjc.202104070187
Citation: Fan Lingfang, Zhang Jingyun, Sha Dan, Guan Hongxia. Application of FilmArray G Panel in the detection of diarrhea pathogens[J]. Disease Surveillance, 2022, 37(6): 807-812. DOI: 10.3784/jbjc.202104070187

FilmArray G Panel在腹泻病原体检测中的应用

Application of FilmArray G Panel in the detection of diarrhea pathogens

  • 摘要:
      目的  探讨FilmArray Gastrointestinal(GⅠ)Panel在腹泻病原体检测中的应用价值。
      方法  收集2016年1-9月无锡市人民医院(含儿童医院)肠道门诊就诊病例粪便标本152份,分别采用FilmArray GⅠ Panel及real-time PCR进行检测,对两种方法检测结果进行比较。
      结果  FilmArray GⅠ Panel共检出19种病原体,阳性检出率为75.00%(114/152);real-time PCR共检出17种病原体,阳性检出率为68.42%(104/152)。 两种方法检测结果符合率67.76%(103/152)。 对腺病毒F组40/41、EIEC(Enteroinvasive E. coli , EIEC)、弯曲菌和蓝氏贾第鞭毛虫,检测结果符合率100.00%;对札如病毒、肠集聚性大肠埃希菌(Enteroaggregative E.coli, EAEC)、艰难梭菌、沙门菌和霍乱弧菌,检测结果符合率为98.03%、94.08%、96.71%、94.08%和98.68%,检测结果一致性中等(Kappa值为0.563、0.546、0.651、0.610、0.744);对诺如病毒GⅠ/GⅡ、A组轮状病毒、肠产毒性大肠埃希菌(Enterotoxigenic E. coli, ETEC)、肠致病性大肠埃希菌(Enteropathogenic E.coli, EPEC)、类志贺邻单胞菌和副溶血性弧菌,检测结果符合率为98.03%、99.34%、97.37%、92.76%、99.34%、98.68%,检测结果一致性较好(Kappa值为0.892、0.971、0.890、0.764、0.944、0.902),经χ2检验分析,FilmArray GⅠ Panel对EAEC、ETEC、EPEC、艰难梭菌和沙门菌检出率高于real-time PCR,差异有统计学意义(P<0.05)。
      结论  与real-time PCR相比,FilmArray检测具有快速、准确、高通量等特点,可用于突发公共卫生事件的快速检测及腹泻病的快速诊断。

     

    Abstract:
      Objective   To discuss the application value of the FilmArray GⅠ Panel in the detection of diarrhea pathogen.
      Methods  152 fecal samplesfrom intestinal outpatients of Wuxi People's Hospital (including Children's Hospital) from January 2016 to September 2016 were collected. FilmArray GⅠ Panel and real-time PCR were used to detect samples, respectively, and the results of the two methods were compared.
      Results  Total of 19 pathogens were detected by FilmArray GⅠ Panel, with a positive rate of 75.00% (114/152). Total of 17 pathogens were detected by real-time PCR detected, with a positive rate of 68.42% (104/152). The coincidence rate between the two methods was 67.76% (103/152).For adenovirus, Enteroinvasive E.coli(EIEC), Campylobacter and G. lambli, the coincidence rate was 100.00%.For Sapovirus, Enteroaggregative E.coli(EAEC), C. difficile, Salmonella and V. cholerae, the coincidence rates were 98.03%, 94.08%, 96.71%, 94.08% and 98.68%, and the consistency was moderate (Kappa values were 0.563, 0.546, 0.651, 0.610, 0.744). For Norovirus GⅠ/GⅡ, Rotavirus A, Enterotoxigenic E.coli(ETEC), Enteropathogenic E.coli(EPEC), P. shigelloides and V. parahaemolyticus, the coincidence rates were 98.03%, 99.34%, 97.37%, 92.76%, 99.34%, 98.68%, and the consistency was good (Kappa values were 0.892, 0.971, 0.890, 0.764, 0.944, 0.902). Using Chi-square tests, the detection rates of EAEC, ETEC, EPEC, C. difficile and Salmonella by FilmArray GⅠ Panel were higher than those by real-time PCR, and the difference was statistically significant (P<0.05).
      Conclusion  Compared with real-time PCR, FilmArray detection has the characteristics of rapid, accurate and high throughput, which can be used for rapid detection of public health emergencies and rapid diagnosis of diarrheal diseases.

     

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