杨晓雯, 姜霞, 安翠红, 孙长云, 李巧玲, 李文博, 蔡英桂, 姜海. 羊种布鲁氏菌分离株体外抗生素敏感性研究[J]. 疾病监测, 2021, 36(12): 1286-1290. DOI: 10.3784/jbjc.202105200283
引用本文: 杨晓雯, 姜霞, 安翠红, 孙长云, 李巧玲, 李文博, 蔡英桂, 姜海. 羊种布鲁氏菌分离株体外抗生素敏感性研究[J]. 疾病监测, 2021, 36(12): 1286-1290. DOI: 10.3784/jbjc.202105200283
Yang Xiaowen, Jiang Xia, An Cuihong, Sun Changyun, Li Qiaoling, Li Wenbo, Cai Yinggui, Jiang Hai. Sensitivity of Brucella melitensis isolates to antibiotics in vitro[J]. Disease Surveillance, 2021, 36(12): 1286-1290. DOI: 10.3784/jbjc.202105200283
Citation: Yang Xiaowen, Jiang Xia, An Cuihong, Sun Changyun, Li Qiaoling, Li Wenbo, Cai Yinggui, Jiang Hai. Sensitivity of Brucella melitensis isolates to antibiotics in vitro[J]. Disease Surveillance, 2021, 36(12): 1286-1290. DOI: 10.3784/jbjc.202105200283

羊种布鲁氏菌分离株体外抗生素敏感性研究

Sensitivity of Brucella melitensis isolates to antibiotics in vitro

  • 摘要:
      目的   为研究布鲁氏菌临床分离株常见抗生素的敏感性。
      方法  本研究利用肉汤稀释法检测布鲁氏菌临床分离株对临床常见布鲁氏菌病治疗药物的最小抑菌浓度(MIC),并利用单因素方差分析比较不同分离省份的菌株药物敏感性差异。
      结果  在现有的布鲁氏菌耐药拐点下,本研究涉及的布鲁氏菌临床分离株常见治疗药物的MIC均在敏感范围内,其中多西环素(0.06~0.12 μg/mL)、四环素(0.06~0.25 μg/mL)、庆大霉素(0.015~0.03 μg/mL)的MIC范围较低;分析分离株氨苄西林MIC值发现,四川省与陕西省(P=0.0001)、河北省(P=0.0001)和湖南省(P=0.04)均差异显著,河北省与广东省(P=0.02)差异显著。另外,本研究还发现了红霉素(32 μg/mL)、复方新诺明(0.25/4.8 μg/mL)MIC值较高的分离株。
      结论  本研究涉及的布鲁氏菌临床分离株对临床常见治疗药物敏感,但部分分离株对复方新诺明等的敏感性较低,应加强监测,防止菌株耐药。 本研究为我国布鲁氏菌耐药水平监测提供基础数据,同时为抗性菌株的筛查及防控提供思路。

     

    Abstract:
      Objective   To study the common antibiotics susceptibility of Brucella isolates.
      Methods   This study used the broth dilution method to detect the minimum inhibitory concentration of clinically common drugs for brucellosis in clinical isolates.
      Results   This study had been found that under the current inflection point of resistance of Brucella, the Brucella isolates were all sensitive to common antibiotics. Among these, the minimum inhibitory concentration value range of doxycycline (0.06−0.12 μg/mL), tetracycline (0.06−0.25 μg/mL) and gentamicin (0.015−0.03 μg/mL) was relatively low; Analyzing the isolated ampicillin MIC values, this study found that Sichuan Province and Shaanxi Province (P=0.0001), Hebei Province (P=0.0001) and Hunan Province (P=0.04) were significantly difference, and Hebei Province and Guangdong Province (P=0.02) were significant difference. In addition, this study also found isolates with higher minimum inhibitory concentration values of erythromycin (32 μg/mL) and compound trimethoprim (0.25/4.8 μg/mL).
      Conclusion   The Brucella isolates in China involved in this study were sensitive to common clinical treatment drugs. However, some isolates were less sensitive to compound trimethoprim. Therefore, follow-up surveillance should be strengthened to prevent bacterial resistance. This study provided basic data for the monitoring of the resistance level of Brucella in China, and at the same time provided ideas for the screening, prevention and control of resistant strains.

     

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