Objective  To investigate the natural infection status of 5 pathogens in rodents in Longyou County of Zhejiang province, and provide evidence for the prevention and control of related diseases.

  Methods  Rodents were captured in the field in Longyou. The lungs of the captured rodents were frozen and fixed in cold acetone. Hanta virus was detected by direct immunofluorescence. Rodent liver DNA extraction was done for the detection of Borrelia burgdorferis, Ehrlichia chaffeensis, Phagocytophilum, and Yersinia pestis by PCR amplification.

  Results  A total of 88 rodents in 6 species were captured in 2017, including Apodemus agrarius, Rattus acutus, Rattus commune, shrew, Rattus flavipectus and Rattus norvegicus. Among them, Apodemus agrarius was the predominant species (88.64%), followed by Rattus acutus (5.00%). In 2019, a total of 103 rodents in 6 species were captured, including Apodemus agrarius, Rattus flavipectus, Rattus acutus, Rattus norvegicus, shrew and nested rat. Apodemus agrarius was still the predominant species, accounting for 84.47% of the total captured rodents. The results of pathogen detection showed that the positive rate of Hanta virus antigen was 3.85% and 4.60% in 2017 and 2019, respectively. The positive rate of Borrelia burgdorferi nucleic acid were 1.28% and 2.30%, respectively. The positive rate of Ehrlichia chaffeensis nucleic acid was 6.41% and 5.75%, respectively. The positive rate of Phagocytophilum nucleic acid was 1.28% and 2.30%, respectively. The detection results of Yersinia pestis were negative. No related pathogens in other species were detected.

  Conclusion  There is a large number of Apodemus agrarius in Longyou, which is a potential infection source of many important infectious diseases. Therefore, it is necessary to strengthen the surveillance for Apodemus agrarius. In addition, the investigation of related diseases should be expanded in the future to provide evidence for the prevention and control of natural focal diseases.