王萌璇, 马鑫, 魏宇航, 彭蕊, 毛彤瑶, 王明雯, 范佳欣, 李金松, 李丹地. 轮状病毒G9P [8] -E2基因型重配株在我国的流行趋势分析[J]. 疾病监测, 2021, 36(8): 780-784. DOI: 10.3784/jbjc.202106210350
引用本文: 王萌璇, 马鑫, 魏宇航, 彭蕊, 毛彤瑶, 王明雯, 范佳欣, 李金松, 李丹地. 轮状病毒G9P [8] -E2基因型重配株在我国的流行趋势分析[J]. 疾病监测, 2021, 36(8): 780-784. DOI: 10.3784/jbjc.202106210350
Wang Mengxuan, Ma Xin, Wei Yuhang, Peng Rui, Mao Tongyao, Wang Mingwen, Fan Jiaxin, Li Jinsong, Li Dandi. Spread trend analysis on reassortant of rotavirus G9P[8]-E2 in China[J]. Disease Surveillance, 2021, 36(8): 780-784. DOI: 10.3784/jbjc.202106210350
Citation: Wang Mengxuan, Ma Xin, Wei Yuhang, Peng Rui, Mao Tongyao, Wang Mingwen, Fan Jiaxin, Li Jinsong, Li Dandi. Spread trend analysis on reassortant of rotavirus G9P[8]-E2 in China[J]. Disease Surveillance, 2021, 36(8): 780-784. DOI: 10.3784/jbjc.202106210350

轮状病毒G9P [8] -E2基因型重配株在我国的流行趋势分析

Spread trend analysis on reassortant of rotavirus G9P8-E2 in China

  • 摘要:
      目的  研究我国轮状病毒(RV)流行毒株G9P[8]基因型的全序列分子特征及进化规律。
      方法  采用反转录–聚合酶链式反应(RT-PCR)方法,对2018年收集的64份(广东省深圳市和吉林省长春市各32份)G9P[8]基因型RV阳性标本进行扩增。
      结果  成功获取54份(深圳市30份, 长春市24份)G9P[8]基因型A组轮状病毒(RVA)标本的全基因组节段。序列分析表明,54份G9P[8]基因型RVA中, 28份为Wa-like G9P[8]-E1株,26份为非结构蛋白NSP4基因节段重配的G9P[8]-E2株,G9P[8]-E2株在深圳和长春市阳性样本中分别占60.0%、33.3%。
      结论  2018年深圳和长春市流行的RVA中G9P[8]-E2重配株已经成为优势株,且在深圳市的优势程度大于在长春市的,推测该基因型重配株RVA可能已在我国广泛传播。

     

    Abstract:
      Objective  To study the full sequence molecular and evolutionary characteristics of rotavirus G9P8 in China.
      Methods  A total of 64 rotavirus G9P8 positive samples collected in 2018 (32 from Shenzhen of Guangdong province and Changchun of Jilin province, respectively) were amplified by reverse transcriptase polymerase chain reaction (RT-PCR).
      Results  The whole genome segments of 54 of rotavirus group A (RVA) G9P8 isolates (30 isolates from Shenzhen and 24 isolates from Changchun) were amplified successfully. Sequence analysis showed that among the 54 RVA G9P8 strains, there were 28 Wa-like strains of G9P8-E1 type (G9-P8-I1-R1-C1-M1-A1-N1-T1-E1-H1), 26 NSP4-segment reassortant G9P8-E2 strains (G9-P8-I1-R1-C1-M1-A1-N1-T1-E2-H1). The reassortant strains in Shenzhen and Changchun accounted for 60.0% and 33.3%, respectively.
      Conclusion  RVA G9P8-E2 reassortant has become the predominant strain in RVA detected in the south and north of China in 2018, which was more obvious in southern China than in northern China. It is speculated that reassortant of RVA G9P8-E2 might have widely spread in China.

     

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