费筱媛, 王华伟, 祁莉, 周军波, 赵滢, 黄烈泓, 龙一兵. 2021年武汉市一起肠炎沙门菌引起的跨区食源性疾病暴发的病原学分析[J]. 疾病监测, 2022, 37(11): 1495-1501. DOI: 10.3784/jbjc.202204140149
引用本文: 费筱媛, 王华伟, 祁莉, 周军波, 赵滢, 黄烈泓, 龙一兵. 2021年武汉市一起肠炎沙门菌引起的跨区食源性疾病暴发的病原学分析[J]. 疾病监测, 2022, 37(11): 1495-1501. DOI: 10.3784/jbjc.202204140149
Fei Xiaoyuan, Wang Huawei, Qi Li, Zhou Junbo, Zhao Ying, Huang Liehong, Long Yibing. Pathogenic analysis on a foodborne disease outbreak caused by Salmonella Enteritidis in Wuhan, 2021[J]. Disease Surveillance, 2022, 37(11): 1495-1501. DOI: 10.3784/jbjc.202204140149
Citation: Fei Xiaoyuan, Wang Huawei, Qi Li, Zhou Junbo, Zhao Ying, Huang Liehong, Long Yibing. Pathogenic analysis on a foodborne disease outbreak caused by Salmonella Enteritidis in Wuhan, 2021[J]. Disease Surveillance, 2022, 37(11): 1495-1501. DOI: 10.3784/jbjc.202204140149

2021年武汉市一起肠炎沙门菌引起的跨区食源性疾病暴发的病原学分析

Pathogenic analysis on a foodborne disease outbreak caused by Salmonella Enteritidis in Wuhan, 2021

  • 摘要:
      目的  对2021年武汉市一起跨区食源性疾病暴发事件进行病原菌的鉴定和溯源分析,查明事件暴发原因。
      方法  采集此次食源性疾病暴发事件中相关人员的肛拭子样本、粪便样本和食品样本进行分离培养,用VITEK 2 Compact 全自动微生物鉴定系统鉴定分离到的菌株,并进行血清学分型,耐药性敏感试验;采用脉冲场凝胶电泳法(PFGE)对本次事件的检出菌及近两年本地区临床散发病例的同型别菌株进行分子分型和聚类分析;提取本次事件病原菌和本地区同型别菌株的核酸进行全基因组测序,通过基因注释和序列比对分析病原菌的毒力因子与耐药基因,结合多位点序列分型,变异位点检测和不同地区同型别菌株的全基因组序列构建基于单核苷酸多态性的系统发育树对病原菌进行溯源。
      结果  共分离出5株肠炎沙门菌,其中2株来自食物样本,3株来自患者粪便和肛拭子样本。 5株检出菌的PFGE带型完全相同,均对抗生素阿米卡星、庆大霉素、妥布霉素、头孢唑啉、头孢西丁、头孢呋辛、甲氧苄啶–磺胺甲恶唑耐药,携带相同的耐药基因和毒力因子,均为ST11型。 本次事件检出菌株的序列间仅存在1个突变位点。 检出菌的全基因组序列与本地肠炎沙门菌序列高度相似。
      结论  肠炎沙门菌为本次事件的致病菌。经同源性分析,此株病原菌为本地区肠炎沙门菌流行株。 全基因组测序技术可作为PFGE分子分型的补充应用于病原菌的监测和暴发事件调查。

     

    Abstract:
      Objective  To identify the cause of a foodborne disease outbreak in Wuhan in 2021.
      Methods  The pathogen was isolated and cultured from the food samples, cases’ anal swabs and stool samples in this foodborne disease. VITEK 2 Compact automatic microbial identification system was used for the identification of isolated strains, and serological typing test and drug resistance sensitivity test were carried for these strains. The nucleic acid of the pathogenic bacteria and the strains with same type isolated sporadically in this area in the past two years were extracted for pulsed field gel electrophoresis (PFGE) and whole genome sequencing, and the virulence genes and drug resistance genes of the strains were analyzed by gene annotation and sequence alignment, combined with multi-locus sequence typing. Phylogenetic tree was constructed based on single nucleotide polymorphism, and homology analysis was performed to trace the origin of pathogenic bacteria.
      Results  A total of 5 strains of Salmonella Enteritidis were isolated, of which 2 were from food samples and 3 were from anal swabs of the cases. The 5 strains had same PFGE pattern and showed resistantance to amikacin, gentamicin, tobramycin, cefazolin, cefoxitin, sulfamethoxazole and cefuroxime. The isolated strains belong to ST11 type, carrying same resistance genes and virulence factors. Only one mutation site was detected in the sequences of the strains isolated in this outbreak. The whole genome sequence of the strains in this outbreak was highly similar to the sequences of the S. Enteritidis strains isolated from local cases.
      Conclusion  This foodborne disease outbreak in Wuhan was caused by S. Enteritidis circulating in this area. Whole genome sequencing can be used as a supplement to pulsed field gel electrophoresis for epidemiological investigation of disease outbreak.

     

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