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覃迪, 陈家良, 贺子翔, 戴志辉, 夏昕, 周海健, 张红, 逄波, 湛志飞. 2018－2021年湖南省人源非O1/非O139群霍乱弧菌药物敏感性及基因组特征[J]. 疾病监测, 2022, 37(11): 1413-1418. DOI: 10.3784/jbjc.202206210282
引用本文: 覃迪, 陈家良, 贺子翔, 戴志辉, 夏昕, 周海健, 张红, 逄波, 湛志飞. 2018－2021年湖南省人源非O1/非O139群霍乱弧菌药物敏感性及基因组特征[J]. 疾病监测, 2022, 37(11): 1413-1418. DOI: 10.3784/jbjc.202206210282
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Qin Di, Chen Jialiang, He Zixiang, Dai Zhihui, Xia Xin, Zhou Haijian, Zhang Hong, Pang Bo, Zhan Zhifei. Drug sensitivity and genomic characterization of non-O1/non-O139 Vibrio cholerae of human isolates from Hunan province, China, 2018−2021[J]. Disease Surveillance, 2022, 37(11): 1413-1418. DOI: 10.3784/jbjc.202206210282
Citation: Qin Di, Chen Jialiang, He Zixiang, Dai Zhihui, Xia Xin, Zhou Haijian, Zhang Hong, Pang Bo, Zhan Zhifei. Drug sensitivity and genomic characterization of non-O1/non-O139 Vibrio cholerae of human isolates from Hunan province, China, 2018−2021[J]. Disease Surveillance, 2022, 37(11): 1413-1418. DOI: 10.3784/jbjc.202206210282
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2018－2021年湖南省人源非O1/非O139群霍乱弧菌药物敏感性及基因组特征

Drug sensitivity and genomic characterization of non-O1/non-O139 Vibrio cholerae of human isolates from Hunan province, China, 2018−2021

    摘要
  • 摘要:
      目的  了解2018—2021年间湖南省从病例分离到的非O1/非O139群霍乱弧菌的药物敏感性及基因组特征。
      方法  选取2018—2021年间湖南省腹泻病例肠道标本分离的非O1/非O139群霍乱弧菌分离株4株和非腹泻病例血液标本分离的非O1/非O139群霍乱弧菌分离株3株，进行药物敏感性测试以及基因组序列测定，分析其药物敏感性及基因组特征。
      结果  3株血液样品分离株对氨苄西林、诺氟沙星、头孢曲松、美罗培南、米诺环素、复方磺胺甲恶唑、庆大霉素、头孢噻肟、萘啶酸、环丙沙星全部敏感，1株对四环素中介，1株对氯霉素耐药；4株粪便样品分离株对诺氟沙星、头孢曲松、美罗培南、米诺环素、复方磺胺甲恶唑、庆大霉素、头孢噻肟全部敏感，1株对四环素、氯霉素中介，1株对氯霉素耐药，2株对氨苄西林、环丙沙星、萘啶酸耐药；其中1株对氨苄西林、萘啶酸、环丙沙星二重耐药；1株对氨苄西林、萘啶酸、环丙沙星、氯霉素多重耐药。 7株非O1/非O139群霍乱弧菌的基因组序列表现出明显的多样性，但是部分菌株在核心基因组和泛基因组都高度相似。 基因组序列中均未检出ctxAB基因、VPI-1和VSPII基因组岛。 分离自粪便的菌株携带耐药基因的数量是4～8个，分离自血液的菌株携带耐药基因的数量是1～2个。
      结论  湖南省非O1/非O139群霍乱弧菌基因组表现出明显的多样性，菌株对多种抗菌药物耐药，需加强腹泻病例、食品及环境水体中非O1/非O139群霍乱弧菌的菌株型别变异及药物敏感性监测。

     

    Abstract:
      Objective  To investigate the drug sensitivity and genomic characteristics of non-O1/non-O139 Vibrio cholerae isolated from patients in Hunan Province during 2018−2021.
      Methods  Four non-O1/non-O139 V. cholerae isolated from intestinal samples of diarrhea cases and three non-O1/non-O139 V. cholerae isolated from blood samples of non-diarrhea cases in Hunan province from 2018 to 2021 were selected for drug sensitivity test and genome sequencing to analyze their drug sensitivity and genomic characteristics.
      Results  Three strains isolated from blood samples were sensitive to ampicillin, norfloxacin, ceftriaxone, meropenem, minocycline, compound sulfamethoxazole, gentamicin, cefotaxime, nalidixicacid and ciprofloxacin, one strain was intermediate to tetracycline, and one strain was resistant to chloramphenicol. The 4 strains isolated from stool samples were sensitive to norfloxacin, ceftriaxone, meropenem, minocycline, compound sulfamethoxazole, gentamicin and cefotaxime. One strain was intermediate to tetracycline and chloramphenicol, one strain was resistant to chloramphenicol, and two strains were resistant to ampicillin, ciprofloxacin and nalidixic acid; among which 1 strain was resistant to ampicillin, nalidixic acid and ciprofloxacin; and 1 strain was resistant to ampicillin, Nalidixic acid, ciprofloxacin and chloramphenicol. The genome sequences of 7 non-O1/non-O139 V. cholerae strains showed significant diversity, but some strains were highly similar in core and pangenome. No ctxAB gene,VPI-1 and VSPII genome islands were detected in the genome sequences. Strains isolated from stool carried 4 to 8 resistance genes, while strains isolated from blood carried 1 to 2 resistance genes.
      Conclusion  The genome of non-O1/non-O139 V. cholerae in Hunan province showed obvious diversity, and the strains were resistant to a variety of antimicrobial agents. It is necessary to strengthen the monitoring of genome variation and drug sensitivity of non-O1/non-O139 V. cholerae in diarrhea cases, food and environmental water.

     

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