田雅昕, 王妙, 李振军, 刘志国, 翟景波. 2004-2021年内蒙古自治区乌兰察布市布鲁氏菌病流行病学及菌株分子特征分析[J]. 疾病监测, 2022, 37(11): 1462-1466. DOI: 10.3784/jbjc.202206270301
引用本文: 田雅昕, 王妙, 李振军, 刘志国, 翟景波. 2004-2021年内蒙古自治区乌兰察布市布鲁氏菌病流行病学及菌株分子特征分析[J]. 疾病监测, 2022, 37(11): 1462-1466. DOI: 10.3784/jbjc.202206270301
Tian Yaxin, Wang Miao, Li Zhenjun, Liu Zhiguo, Zhai Jingbo. Molecular characteristics of Brucella strains and epidemiological characteristics of human brucellosis in Ulanqab, Inner Mongolia, 2004−2021[J]. Disease Surveillance, 2022, 37(11): 1462-1466. DOI: 10.3784/jbjc.202206270301
Citation: Tian Yaxin, Wang Miao, Li Zhenjun, Liu Zhiguo, Zhai Jingbo. Molecular characteristics of Brucella strains and epidemiological characteristics of human brucellosis in Ulanqab, Inner Mongolia, 2004−2021[J]. Disease Surveillance, 2022, 37(11): 1462-1466. DOI: 10.3784/jbjc.202206270301

2004-2021年内蒙古自治区乌兰察布市布鲁氏菌病流行病学及菌株分子特征分析

Molecular characteristics of Brucella strains and epidemiological characteristics of human brucellosis in Ulanqab, Inner Mongolia, 2004−2021

  • 摘要:
      目的  对2004—2021年内蒙古自治区乌兰察布市人间布鲁氏菌病(布病)的流行特征进行分析,为制定该地区人间布病防控策略提供理论支持。
      方法  采用发病数、发病率和构成比等描述疫情。 采用AMOS-聚合酶链式反应(PCR)和多位点可变数目串联重复序列分析(MLVA)对 2016年该地区分离的菌株进行鉴定和基因分型。 基于MLVA-16分型方法对本研究的菌株与国际布鲁氏菌MLVA数据库的羊种布鲁氏菌进行比较,调查菌株间的分子流行病学关联。
      结果  2004—2011年乌兰察布市人间布病呈现逐年增多态势,并在2011年到达流行顶峰, 2012—2108年显著下降,但2019—2021年再次反弹。 2004—2021年该地区共报告人间布病29 713例,年均报告1 650例,年均发病率为75.46/10万。 乌兰察布市后山地区的流行情况高于前山地区。 经AMOS-PCR和MLVA-8两种分子方法鉴定表明,22株菌均为羊种布鲁氏菌,且属于东地中海血统。 菌株间有较高的遗传相似性(80%~100%),提示菌株来自单一的共同祖先。 此外,来自本研究的20个株菌分别与先前该地区分离菌株和该地区之外的菌株形成完全相同的MLVA-16基因型,表明菌株持续在本地区传播扩散,且呈现跨地区或跨国界传播特点。
      结论  乌兰察布市人间布病疫情得到一定控制,但流行形势仍较为严重,存在疫情输入和(或)输出风险,应加强传染源(疫羊)的管控,警惕再次反弹。

     

    Abstract:
      Objective  To analyze the epidemiological characteristics of human brucellosis in Ulanqab, Inner Mongolia, from 2004 to 2021, and provide theoretical support for the development of human brucellosis prevention and control strategies in this area.
      Methods  The number of cases, incidence rate, and composition ratio of human brucellosis were used to describe the characteristics of the epidemic. The strains isolated in 2016 in this region were identified and genoryping by AMOS-PCR and MLVA assay. Moreover, based on the MLVA-16 typing approach, the strains in this study were compared with Brucella melitensis from the international Brucella MLVA database to investigate the molecular epidemiological relationship between the strains.
      Results  The prevalence of human brucellosis in Ulanqab increased year by year from 2004 to 2011, reaching its peak in 2011, showed a decline trend from 2012 to 2108 and increased again during 2019−2021. From 2004 to 2021, a total of 29 713 human brucellosis cases were reported in this area, with 1,650 cases being reported in each year and an average annual incidence rate of 75.46/100 000. The prevalence of human brucellosis in the back mountain area of Ulanqab was significantly higher than that in the front mountain area. The identification by two molecular methods (AMOS-PCR and MLVA-8) showed that the 22 strains isolated were all Brucella melitensis, and the strains belonged to the Eastern Mediterranean lineage. These strains had high genetic similarity (80%−100%), suggesting a single common ancestor. Likewise, the 20 strains from this study had complete identical MLVA-16 genotype with the previous isolates in this area and in other areas, respectively, indicating that the strains can not only spread in the area in the future but also has the characteristics of cross-regional or cross-border spread.
      Conclusion  The human brucellosis epidemic in Ulanqab has been controlled to a certain level, but the epidemic is still serious. There is a risk of epidemic importation or exportation, so it is suggested to strengthen the control of the source of infection (infected sheep) for the prevention of human brucellosis.

     

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