袁梦, 李博, 程妍, 袁月明. 一起副溶血性弧菌引起食物中毒实验室溯源检测[J]. 疾病监测, 2012, 27(3): 214-217.
引用本文: 袁梦, 李博, 程妍, 袁月明. 一起副溶血性弧菌引起食物中毒实验室溯源检测[J]. 疾病监测, 2012, 27(3): 214-217.
YUAN Meng, LI Bo, CHENG Yan, YUAN Yue-ming. Laboratory detection of food poisoning caused by Vibrio parahaemolyticus[J]. Disease Surveillance, 2012, 27(3): 214-217.
Citation: YUAN Meng, LI Bo, CHENG Yan, YUAN Yue-ming. Laboratory detection of food poisoning caused by Vibrio parahaemolyticus[J]. Disease Surveillance, 2012, 27(3): 214-217.

一起副溶血性弧菌引起食物中毒实验室溯源检测

Laboratory detection of food poisoning caused by Vibrio parahaemolyticus

  • 摘要: 目的 分析一起食物中毒中分离的副溶血性弧菌(Vibrio parahaemolyticus,Vp)的血清群分布、毒力基因携带情况及分子分型特征,为溯源提供依据。 方法 血清凝集试验检测菌株血清型别,多重PCR方法检测Vp三种毒力基因tdh(耐热直接溶血素),trh(相对耐热直接溶血素)与tlh(不耐热溶血素)的携带情况。肠道细菌重复基因间共同序列(enterobacterial repetitive intergenic consensus sequence,ERIC)PCR分型技术分析不同来源菌株基因型特征。 结果 24株分离株中主要血清群为O3:K6(33.3%),均携带tlh基因、均不携带trh基因。66.7%(16/24)的菌株携带tdh基因,O3:K6血清群菌株均携带tdh基因。ERIC PCR分型分为3个克隆群:E1群包括4株O1,2株O2,O4、O5各1株,E2群包括3株O1,2株O5,O2、O4、O10各1株;E3群包括8株O3。 结论 该起食物中毒是由多种型别Vp引起。主要血清群为O3:K6,在食品与患者中都分离出该血清群的菌株,经ERIC分型,这些菌株来自同一个克隆群。除O3血清群外,从食品、加工存放食品的器具及患者中分离出其他不同血清群Vp,经ERIC分型,不同血清群分为2大克隆群,提示此次食物中毒的传染源可能与食品、加工存放食品器具污染Vp相关。

     

    Abstract: Objective To understand the serotypes, virulence genes and molecular types of Vibrio parahaemolyticus causing a food poisoning event and provide evidence for the infection source identification. Methods Serotypes, three genes (tlh,tdh,tdh) of V. parahaemolyticus strains were detected by serum agglutination and multiplex polymerase chain reaction (PCR) respectively. The molecular types were detected by enterobacterial repetitive intergenic consensus sequence (ERIC) PCR. Results O3:K6 (33.3%) was the predominant serogroup. All V. parahaemolyticus strains were positive for tlh gene and negative for trh gene. Totally 66.7% of V. parahaemolyticus strains were positive for tdh gene, all O3:K6 serogroup strains were positive for tdh gene. Three clone clusters were typed by ERIC PCR. E1 clone cluster included 4 serogroup O1 strain, 2 serogroup O2 strains, 1 serogroup O4 strain and 1 serogroup O5 strain. E2 clone cluster included 3 serogroup O1 strains, 2 serogroup O5 strains, 1 serogroup O2 strain, 1 serogroup O4 strain and 1 serogroup O10 strain. E3 clone cluster included 8 serogroup O3 strains. Conclusion The food poisoning was caused by several serogroups of V. parahaemolyticus. O3:K6 was the major serogroup, which were isolated from food and patient samples and in same clone cluster by ERIC PCR. E1 and E2 clone clusters included all serogroups except O3. The laboratory detection indicated that the food poisoning was associated with the contamination of foods by several serogroups of V. parahaemolyticus.

     

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