Abstract: Objective To understand the prevalence and drug-resistance of Helicobacter pylori (H.pylori) in gastropathic patients in Huzhou. Methods The patients gastric mucosa tissue were collected for the isolation and culture of H.pylori. The suspected bacteria colonies were microscopic examined after smearing and identified by biochemical test. The drug susceptibility test was conducted by K-B method. Results A total of 902 specimens of patients gastric mucosa tissue were examined, and 354 strains of H. pylori were detected (39.25%). The positive rate was 66.67% in gastric cancer patients (16/24),40.08% in gastritis patients (295/736),36.58% in gastric ulcer patients (30/82),21.67% in other gastropathic patients (13/60).The prevalence of H. pylori in gastric cancer patients was significantly higher than others (P0.05), and the difference on the prevalence of H. pylori between males (39.96%) and females (37.64%) was not statistically significant. The drug susceptibility test were performed for the 354 H. pylori strains, the results indicated that the H. pylori were sensitive to amoxicillin, gentamicin and furazolidone, whereas the drug resistant rate was 8.19% to levofloxacin (29/354),19.21% to clarithromycin (68/354) and 100% to metronidazole (354/354), the multi drug resistant rate was 16.95% to clarithromycin and metronidazole (60/354),4.24% to levofloxacin and metronidazole (15/354) and 1.98% to clarithromycin, levofloxacin and metronidazole (7/354). Conclusion H. pylori infection is closely related to gastric diseases and mainly occurs in age group of 45-55 years. Close attention should be paid to the drug-resistance of levofloxacin, clarithromycin and metronidazole. H. pylori infection in Huzhou could be treated by using amoxicillin, gentamycin and furazolidone, but not by metronidazole.