耐头孢西丁肺炎克雷伯菌ESBLs和AmpC酶的检测及耐药基因分析
Detection of ESBLs and AmpC enzyme in Cefoxitin-resistant Klebsiella pneumoniae and analysis of drug-resistant genotype
-
摘要: 目的 了解浙江省台州地区肺炎克雷伯菌ESBLs和AmpC -内酰胺酶产生情况及AmpC酶的耐药基因型别特征。 方法 采用VITEK-AMS60全自动细菌鉴定仪鉴定细菌,按CLSI推荐的确证试验检测ESBLs,采用头孢西丁纸片扩散法筛选疑产AmpC酶阳性菌株,通过酶粗提物三维试验确证产AmpC酶,并经PCR测序等实验分析该菌株的基因型。 结果 在对头孢西丁不敏感的86株肺炎克雷伯菌中,ESBLs和AmpC酶检出率分别为88.37%和77.91%;以同产ESBLs和AmpC酶为主要形式,占47.67%,单产ESBLs和单产AmpC酶分别占40.70%和30.23%;AmpC酶阳性菌株的耐药基因型:62株为DHA-1型、5株为ACT-1型。 结论 台州地区肺炎克雷伯菌产ESBLs和AmpC酶检出率较高,AmpC酶以DHA-1型为主。Abstract: Objective To understand the producing of ESBLs and AmpC -lactamase of Klebsiella pneumoniae and the drug-resistant phenotype characteristics of AmpC enzyme in Taizhou. Methods Bacteria were identified with VITEK-AMS60. ESBLs were detected with the confirmatory test recommended by CLSI. Positive strains harboring AmpC enzyme were screened with cefoxitin disk diffusion. Methods AmpC enzyme was confirmed by a three-dimensional test and genotype was differentiated by PCR-sequencing. Results In the 86 isolates of Klebsiella pneumoniae insensitive to cefoxitin, the detection rates of ESBLs and AmpC enzyme were 88.37% and 77.91% respectively. Among them, 47.67% produced both ESBLs and AmpC enzyme, 40.70% produced only ESBLs and 30.23% produced only AmpC enzyme. The drug-resistant genotypes for AmpC-positive strains were DHA-1(62 isolates) and ACT-1(5 isolates). Conclusion The detection rate for Klebsiella pneumoniae haboring ESBLs and AmpC enzyme was high in Taizhou and AmpC enzyme mainly belonged to DHA-1 genotype.
下载: