严春, 钱定良, 詹爱霞, 李克诚. 多重耐药鲍曼不动杆菌携带碳青霉烯类水解酶基因情况的研究[J]. 疾病监测, 2011, 26(9): 736-738.
引用本文: 严春, 钱定良, 詹爱霞, 李克诚. 多重耐药鲍曼不动杆菌携带碳青霉烯类水解酶基因情况的研究[J]. 疾病监测, 2011, 26(9): 736-738.
YAN Chun, QIAN Ding-liang, ZHAN Ai-xia, LI Ke-cheng. Hydrolysis enzyme of carbapenems carrying in multidrug-resistant Acinetobacter baumannii[J]. Disease Surveillance, 2011, 26(9): 736-738.
Citation: YAN Chun, QIAN Ding-liang, ZHAN Ai-xia, LI Ke-cheng. Hydrolysis enzyme of carbapenems carrying in multidrug-resistant Acinetobacter baumannii[J]. Disease Surveillance, 2011, 26(9): 736-738.

多重耐药鲍曼不动杆菌携带碳青霉烯类水解酶基因情况的研究

Hydrolysis enzyme of carbapenems carrying in multidrug-resistant Acinetobacter baumannii

  • 摘要: 目的 研究浙江省瑞安市人民医院多重耐药鲍曼不动杆菌中金属-内酰胺酶(IMP、VIM)、 KPC酶、 OXA-23型水解酶基因的携带情况,探讨各种碳青霉烯类水解酶在多重耐药鲍曼不动杆菌中的作用。 方法 采用Whonet 5.4 软件分析多重耐药鲍曼不动杆菌的标本类型和病区分布,以及药敏资料;设计特异性引物,用PCR方法扩增IMP、VIM、KPC和OXA-23特异基因,并用琼脂糖电泳分析其产物。 结果 70株多重耐药鲍曼不动杆菌均未检测到IMP、VIM及KPC基因,其中58株亚胺培南耐药菌株均携带OXA-23基因,阳性率为82.86%。 结论 OXA-23型水解酶是造成瑞安市人民医院鲍曼不动杆菌对临床常用碳青霉烯类抗生素耐药的主要原因。

     

    Abstract: Objective To understand the carriage of Metal -lactamase (IMP, VIM), KPC enzyme, OXA-23 type hydrolysis enzyme in multidrug-resistant Acinetobacter baumannii in our hospital and explore the effect of these hydrolysis enzyme in carbapenems antibiotic-resistant bacteria. Methods By using Whonet 5.4, the distribution and drug susceptibility data of multidrug-resistant Acinetobacter baumannii were analyzed. By designing the specific primers, IMP, VIM, KPC and OXA-23 genes were amplified by PCR and their products were analyzed by agarose electrophoresis. Results OXA-23 gene was detected in 58 of 70 strains of multidrug-resistant Acinetobacter baumannii, the positive rate was 82.86%. No IMP,VIM and KPC genes were detected. Conclusion OXA-23 type hydrolysis enzyme was an important factor to cause the common clinical carbapenems antibiotic-resistance of Acinetobacter baumannii in our hospital.

     

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