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陈锡欣, 黄炳成, 王福勇. 32P与光敏生物素标记DNA探针在疟疾监测中的应用研究[J]. 疾病监测, 1995, 10(2): 41-44. DOI: 10.3784/j.issn.1003-9961.1995.2.41
引用本文: 陈锡欣, 黄炳成, 王福勇. 32P与光敏生物素标记DNA探针在疟疾监测中的应用研究[J]. 疾病监测, 1995, 10(2): 41-44. DOI: 10.3784/j.issn.1003-9961.1995.2.41
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Chen Xixin. et al, . Studies on the Application of α—p—Datp of Bio—11—Dutp—labelled Pbf DNA Probe in the Surveilolace of Malaria[J]. Disease Surveillance, 1995, 10(2): 41-44. DOI: 10.3784/j.issn.1003-9961.1995.2.41
Citation: Chen Xixin. et al, . Studies on the Application of α—p—Datp of Bio—11—Dutp—labelled Pbf DNA Probe in the Surveilolace of Malaria[J]. Disease Surveillance, 1995, 10(2): 41-44. DOI: 10.3784/j.issn.1003-9961.1995.2.41
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32P与光敏生物素标记DNA探针在疟疾监测中的应用研究

Studies on the Application of α—p—Datp of Bio—11—Dutp—labelled Pbf DNA Probe in the Surveilolace of Malaria

    摘要
  • 摘要: 本文报告了应用３２Ｐ和光敏生物素标记克隆的ｐＢＦ４ＤＮＡ，作为探针，以斑点杂交试验，检测海南、云南和山东省的血样。两种标记探检测结果与镜检的符合率分别为恶性疟９８．６％和９６．２％；间日疟９１．６％和８６．７％；正常人血９５．２％和１００％；并可从多种疟原虫标本中，特异地检出恶性疟原虫，显示探针具有恶生疟原虫的种特异性。检测的敏感度为８４个原虫／μｌ血，检测原虫ＤＮＡ可达１０ｐｇ水平。表明探针具有良好的实用性。

     

    Abstract: This report deals with cloned Pbf DNA probe from the genomic DNA library of plas-modium falaiparum labelled with-p-Datp of BIO-11-Dutp was used in dot hybridization to detect blood samples from Hainan,Yunnan and Shandong.The results have shown the coincidence rate of de-tection between those probe and with microscope respectively were 98.6%,96.2%for Plasmodium falciparum;91.6%,86.7% for Plasmodium vivax and 95.2%,100% for normal controls.Plasm modium falciparum could be identified specifically from samples with various malaria parasites,indi-cating that these probe have high specificity for falciparum maolaria infecton.The parasites as low as 84ul blood or 10 pg of parasite DNA could still be discriminated.

     

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