Abstract: ObjectiveTo investigate the distribution of infectious pathogens in respiratory disease department and analyze their drug resistance, provide evidence to guide clinical medication and control nosocomial infections. MethodsThe pathogens isolated from patients in our hospital from January 2007 to December 2008 were identified and the drug susceptibility tests were performed. ResultsA total of 1501 strains of pathogens were isolated, of which 841 were gram negative bacilli strains (56.03%); 291 were gram positive coccus strains (19.39%); 369 were fungus strains (24.58%). Among the gram negative bacilli strains, the most common pathogens were Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterobacter cloacae, Escherichia coli, Acinetobacter baumannii and Haemophilus influenzae, and among the gram positive coccus strains, the most common pathogens were Streptococcus penumoniae, Staphylococcus aureus and microbacterium. The drug susceptibility test results indicated that Klebsiella pneumoniae, Enterobacter cloacae, Escherichia coli and Acinetobacter baumannii were 100% sensitive to imipenem and over 90% sensitive to cefperazone-sulbactam; Pseudomonas aeruginosa was resistant to common antibiotics at different degree. The sensitive rate of Streptococcus penumoniae to penicillin was 43.75%; no strains of Staphylococcus aureus and Enterococcus faecalis or Enterococcus faecium were found to be resistant to vancomycin. ConclusionThe drug resistance of the nosocomial infection pathogens now is more serious. Its important to conduct the surveillance of pathogen drug resistance regularly for the rational use of antibiotics and effective control of nosocomial infections.