Abstract: Objective To investigate the distribution and drug resistance of Pseudomonas aeruginosa in our hospital during past three years and provide evidence for the rational use of antibiotics. Methods A total of 2162 strains of P. aeruginosa were isolated from the specimens of patients hospitalized in our hospital from January 2011 to December 2013, and their drug resistance was analyzed retrospectively by using WHONET 5.5 software. Results Of 2162 strains of P. Aeruginosa, 1583 (73.22%) were isolated from respiratory tract samples. The strains mainly detected from the patients in ICU (27.80%) and respiratory disease department (20.58%). The isolated strains showed the high drug resistant rate to cefotaxime (67.30%) and ceftriaxone (62.12%) and the low drug resistant rate to cefoperazone/sulbactam. The drug resistance rates to imipenem was 32.10%. The detection rate of multi drug resistant P. Aeruginosa and extensive drug resistant P. Aeruginosa were 13.51% and 5.50% respectively. Conclusion P. aeruginosa infection mainly occurred in the patients in ICU and respiratory disease department, and the monitoring of drug resistance of P. aeruginosa should be strengthened to control P. Aeruginosa infection and guide the rational use of antibiotics.