浙江省丽水市监测点鼠类恙虫病东方体的检测

Detection of Rickettsiae tsutsugamushi in rodents in Lishui city of Zhejiang province

摘要: 目的对丽水市莲都区鼠肝脾组织进行恙虫病东方体(Ot)检测,了解该区鼠恙虫病感染情况。方法收集丽水市鼠的肝脾标本,采用巢式PCR法检测56 kD蛋白基因,确定阳性标本。结果在丽水莲都区捕获鼠132只,在黑线姬鼠中检测到Ot阳性标本3份,在小家鼠中检测到Ot阳性标本1份,鼠Ot总阳性率为3.03%(4/132)。结论丽水市鼠中存在Ot自然感染,且宿主动物呈多样性,为深入进行恙虫病疫源地的调查奠定了基础。

Abstract: Objective To investigate the natural infection status of Rickettsiae tsutsugamushi in rodents in Lishui, and provide scientific evidence for the prevention and control of the disease. Methods The liver and spleen specimens of the rodents captured in Lishui were collected to detect the 56 kD gene by nested polymerase chain reaction (nPCR). Results Totally 132 rodents were captured in Lishui. Three specimens of Apodemus agrarius and 1 specimen of Mus musculus were positive for Rickettsiae tsutsugamushi. The positive rate was 3.03% (4/132). Conclusion The finding indicated that Rickettsiae tsutsugamushi infection do exists in rodents in Lishui and the host animals are diversified, which could be used in the further investigation of tsutsugamushi focus in Lishui.