Abstract: Objective To understand the distribution and drug resistance of non-fermentative bacteria in patients with urinary tract infection and provide the reference for the rational clinical use of antibiotics. Methods The retrospective survey of drug susceptibility of non-fermentative bacterium strains isolated from the urine specimens of the patients with urinary tract infection in our hospital from 2008 to 2012 and cultured with Uricult. The K-B method was adopted in drug susceptibility test and the result was analyzed with Whonet 5.6 software. Results A total of 146 strains were isolated,in which 56 were Pseudomonas aeruginosa strains (38.4%), 36 were Acinetobacter baumannii strains (24.7%) and 16 were Acinetobacter lwoffii (11.0%). The strains were mainly isolated from the patients in urinary surgery section (32.9%) and internal medicine section (24.7%). The resistant rate of Pseudomonas aeruginosa was 100% to ampicillin, 19.6% to imipenem and 18.4% to meropenem. The resistant rate of Acinetobacter baumannii was 97.2% to ampicillin, 69.0% to cefotaxime, 13.9% to imipenem and 20.0% to meropenem. Pseudomonas aeruginosa and Acinetobacter baumannii showed low resistant rate to enzyme inhibitor cefoperazone/Sulbactam. Conclusion Pseudomonas aeruginosa was the predominant pathogen in the patients with urinary tract infection caused by non-fermentative bacteria; Cefoperazone/sulbactam are suitable for the empiric treatment of urinary tract infection. Regular monitoring and analysis of the distribution and drug resistance of urinary tract infection pathogens would facilitate the improvement of empiric treatment and clinical drug use.