Abstract: Objective To understand the health status of non-remunerated blood donors, provide evidence for the recruitment of low-risk blood donors and ensure the safety of blood transfusion. Methods The retrospective analysis was conducted on the detection results of non-remunerated blood donors in Jinhua in recent 5 years. Results Among 215 827 blood donors detected, 2475 were alanine aminotransferase (ALT) positive (1.01%), 1534 were HBsAg positive (0.63%), 489 were anti HCV positive (0.20%), 385 responded in anti-HIV detection (0.16%), in which 26 were confirmed to be anti-HIV positive (0.012%), and 1203 were anti-TP positive (0.49%). The overall ALT, HBsAg and anti HCV positive rates changed from downward to upward trend. The overall anti-HIV and anti-TP positive rates were in a up and down fluctuating. The differences were statistically significant (PConclusion Increased ALT was the major factor influencing blood quality, it is necessary to strengthen the ALT screening among the blood donors who are overweight and with abnormal BMI, especially in hot season. It is better to use more sensitive colloidal gold strip for HBsAg detection and colloidal gold strip for TP rapid screening. Meanwhile, the quality of the detection of other blood borne infectious disease markers should be strengthened to improve blood quality and minimize risk of blood transfusion.