Abstract: Objective To understand the hypo-type distribution of human papilloma virus (HPV) in infected women and its relationship with age in Huzhou, Zhejiang province, and provide evidence for the cervical cancer prevention and control in local women and the development of HPV vaccine. Methods HPV-DNA detection was conducted by using cervical exfoliated cell samples collected from 10 680 females seeking medical care in our hospital from January 2011 to December 2013. Results Among the 10 680 samples, 2865 were detected to be HPV positive (26.83%). The HPV hypo-type detection indicated that the first 3 types were HPV16 (17.52%), HPV52 (15.32%) and HPV58 (12.42%). The high risk infection accounted for 82.41% (2361/2865), and the low risk infection accounted for 8.90% (255/2865). The high/low risk co-infection accounted for 8.69% (249/2865). The infection rates of low risk type, high risk type and co-infection rate of high/low risk type were highest in age group20 years. The simplex type infection accounted for 69.56%. The simplex type infection rate was highest in age group 45-49 years (77.56%), the multi type infection rate was highest in age group20 years (60.38%). Conclusion The HPV infection rate was 26.83% among the females seeking medical care in our hospital. HPV16, 18 and 52 were predominant. The high infection rate and high multi type infection rate in age group 20 years suggested the younger age trend and multi type trend of HPV infection, to which much attention should be paid.