银色棒状杆菌健康携带株耐药性及毒力基因分析

连星星; 秦天; 董魁; 李振军; 杨才新; 蒋艳; 李银妹; 杨晶; 徐建国

doi:10.3784/jbjc.202201130687

银色棒状杆菌健康携带株耐药性及毒力基因分析

Corynebacterium argentoratense isolated from the pharyngeal microbiota of human

摘要

摘要:
目的首次从健康人呼吸道分离到银色棒状杆菌，可进一步探究这一细菌的耐药性和致病性获得此种细菌更加全面的信息。
方法采用生化鉴定和16S rRNA全长基因序列分析法对2020年大学生健康体检中采集的咽拭子样本进行银色棒状杆菌的分离鉴定，同时对鉴定菌株进行抗生素耐药性测定、基因组测序和耐药基因、毒力基因分析。
结果从717份健康大学生咽拭子分离到5株银色棒状杆菌。药敏结果表明这5株菌均对青霉素、莫西沙星、庆大霉素耐药；4株菌对环丙沙星、克林霉素耐药，存在多重耐药情况。基因组序列分析发现，对克林霉素耐药的菌株均含有耐药基因erm(X)，分离菌株携带有Sigma A、GroEL(Hsp60)/Cpn60.2、Nucleoside diphosphate kinase（NDK）、EF-Tu、 MprA/B、 DtxR以及Irp6和 HmuTUV等毒力因子，致病性分析结果表明5株银色棒状杆菌均携带glyA1、mihF、CarD等10个与结核分枝杆菌的毒力密切相关的基因。
结论首次从健康人咽部分离到银色棒状杆菌，其携带耐药及毒力基因。

Abstract:
Objective For the first time, Corynebacterium argentoratense was isolated from the respiratory tract of healthy people, and the drug resistance and pathogenicity of this bacterium can be further explored to obtain more comprehensive information on this bacterium .
Methods Biochemical identification and 16S rRNA full-length gene sequence analysis were used to isolate and identify C.argentoratense from throat swab samples collected during health examinations of college students in 2020. Meanwhile, antibiotic resistance, genome sequencing, drug resistance gene, and virulence gene analysis of the identified strains were conducted.
Results Five strains of C. argentoratense were isolated from 717 pharyngeal swabs of healthy college students. Drug sensitivity results showed that all 5 strains were resistant to penicillin, moxifloxacin, and gentamicin. Four strains were resistant to ciprofloxacin and clindamycin, with multiple resistance. Genome sequence analysis showed that clindamycin-resistant strains all contained drug resistance gene erm(X). The isolates carried virulence factors including Sigma A, GroEL(Hsp60)/Cpn60.2, Nucleoside diphosphate kinase (NDK), EF-Tu, MprA/B, DtxR, Irp6, and HmuTUV. The pathogenicity analysis showed that all 5 strains of C. argentoratense carried 10 genes, including glyA1, mihF, and CarD, which were closely related to the virulence of M. tuberculosis.
Conclusion For the first time, C. argentoratense was isolated from the pharynx of healthy people, and it carried drug resistance and virulence genes.

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