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刘美玲, 李鑫鸽, 宁一桦, 罗昶, 杨超杰, 杜昕颖, 王琪, 邱少富, 奉水东, 向莹. 2008－2018年北京市人源鼠伤寒沙门菌的耐药与分子分型研究[J]. 疾病监测, 2022, 37(4): 523-530. DOI: 10.3784/jbjc.202203010077
引用本文: 刘美玲, 李鑫鸽, 宁一桦, 罗昶, 杨超杰, 杜昕颖, 王琪, 邱少富, 奉水东, 向莹. 2008－2018年北京市人源鼠伤寒沙门菌的耐药与分子分型研究[J]. 疾病监测, 2022, 37(4): 523-530. DOI: 10.3784/jbjc.202203010077
shu
Liu Meiling, Li Xinge, Ning Yihua, Luo Chang, Yang Chaojie, Du Xinying, Wang Qi, Qiu Shaofu, Feng Shuidong, Xiang Ying. Antibiotic resistance and molecular epidemiological characteristics of Salmonella Typhimurium from human in Beijing, 2008−2018[J]. Disease Surveillance, 2022, 37(4): 523-530. DOI: 10.3784/jbjc.202203010077
Citation: Liu Meiling, Li Xinge, Ning Yihua, Luo Chang, Yang Chaojie, Du Xinying, Wang Qi, Qiu Shaofu, Feng Shuidong, Xiang Ying. Antibiotic resistance and molecular epidemiological characteristics of Salmonella Typhimurium from human in Beijing, 2008−2018[J]. Disease Surveillance, 2022, 37(4): 523-530. DOI: 10.3784/jbjc.202203010077
shu

2008－2018年北京市人源鼠伤寒沙门菌的耐药与分子分型研究

Antibiotic resistance and molecular epidemiological characteristics of Salmonella Typhimurium from human in Beijing, 2008−2018

    摘要
  • 摘要:
      目的  分析2008—2018年北京市人源鼠伤寒沙门菌的耐药特征及分子分型特征。
      方法  基于肠道病原监测平台收集的分离于2008—2018年的109株人源鼠伤寒沙门菌，采用生化检测试剂条和血清凝集试验进行菌株复核，采用微量肉汤法进行药敏试验，通过聚合酶链式反应方法检测β-内酰胺类、喹诺酮类和大环内酯类药物相关耐药基因，并通过脉冲场凝胶电泳（PFGE）进行分子分型分析。
      结果  北京市鼠伤寒沙门菌对传统抗生素普遍耐药，多重耐药率达52.29%，对临床一线推荐药物环丙沙星、头孢类药物和阿奇霉素的耐药率分别为21.10%，17.43%和1.83%。 还发现了对头孢类药物和环丙沙星共同耐药以及对头孢类药物和阿奇霉素共同耐药的菌株。 耐药基因检测结果显示，耐头孢类药物菌株中，最常见的超广谱β-内酰胺酶基因是blaTEM-1，其次是blaCTX-M-14，同时也首次于鼠伤寒沙门菌中发现了blaOXA-29基因的携带。 环丙沙星耐药主要与gyrA、parC和parE基因的点突变有关。 耐阿奇霉素菌株中未检测出相关耐药基因。PFGE分子分型结果显示，109株菌可分为67个带型，存在包含13株菌的优势带型。
      结论  北京市人源鼠伤寒沙门菌耐药形势严峻，PFGE图谱呈多态性分布，相同PFGE带型菌株间具有更相似的耐药谱，需进一步加强肠道病原菌的耐药监测以有效控制耐药性的传播扩散。

     

    Abstract:
      Objective  To analyze the antibiotic resistance and molecular characteristics of Salmonella Typhimurium strains isolated from human stool samples in Beijing from 2008 to 2018.
      Methods  Biochemical detection and serum agglutination test were conducted to verify 109 strains of S. Typhimurium isolated in Beijing from 2008 to 2018 and collected by the intestinal pathogen surveillance platform. The antibiotic susceptibility test was carried out by broth method. The β-lactams, quinolones and macrolides resistant genes were detected by polymerase chain reaction (PCR). The molecular typing was conducted by pulsed-field gel electrophoresis (PFGE).
      Results  The S. Typhimurium strains isolated in Beijing were generally resistant to traditional antibiotics, and 52.29% were multidrug-resistant strains. The resistance rate to the three most important front-line antibiotics (ceftriaxone, ciprofloxacin, and azithromycin) were 21.10%, 17.43%, and 1.83% respectively. We also found that the strains were co-resistant to cephalosporins and ciprofloxacin and co-resistant to cephalosporins and azithromycin. The results of antibiotic resistance gene detection showed that the most common β-lactams gene of cephalosporin-resistant strains was blaTEM-1, followed by blaCTX-M-14, and blaOXA-29 gene was firstly identified in S. Typhimurium. The gene point mutations mainly led to ciprofloxacin resistance, including gyrA, parC, and parE genes. No azithromycin resistance-related genes were detected in azithromycin-resistant strains. The results of PFGE molecular typing showed that the 109 strains could be divided into 67 bands, and a predominant band including 13 strains was found.
      Conclusion  The antibiotic resistance of S. Typhimurium strains isolated from human stool samples in Beijing was serious. The PFGE pattern showed a polymorphic distribution, and strains with the same PFGE band had a more similar antibiotic resistance spectrum. It is necessary to further strengthen the surveillance of antibiotic resistance of intestinal pathogens for the effective control of the spread of antibiotic resistance.

     

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