2022年浙江省宁波市1例恙虫病东方体Boryong基因亚型感染病例调查分析

徐可叶; 平国华

doi:10.3784/jbjc.202302160045

2022年浙江省宁波市1例恙虫病东方体Boryong基因亚型感染病例调查分析

Investigation of an infection case of Orientia tsutsugamushi Boryong sub-genotype in Ningbo, Zhejiang, 2022

摘要

摘要:
目的对2022年1例恙虫病疑似病例进行实验室检测和分析，了解浙江省宁波市恙虫病东方体基因型流行情况。
方法对疑似病例进行流行病学调查，询问其病史；采集病例发热期血液，提取总DNA采用实时荧光定量PCR方法扩增目的基因tsa56，根据测得的序列构建系统进化树，确定该恙虫病东方体基因型。
结果实时荧光定量PCR方法检出病例血液中存在恙虫病东方体；扩增序列经比对后与已知恙虫病东方体Boryong具有最近亲缘关系。
结论宁波市实验室确诊1例恙虫病东方体感染病例，为Boryong基因亚型。

Abstract:
Objective To understand the genotype of Orientia tsutsugamushi by analyzing a suspected tsutsugamushi disease case detected in Ningbo, Zhejiang province, 2022.
Methods Epidemiological investigation was conducted. Blood samples of the case in fever phase were collected for the extraction of pathogen DNA. Quantitative real-time fluorescent PCR was performed for the amplification of targeted gene tsa56, and a phylogenetic tree was constructed based on gene sequencing result to determine the genotype of O. tsutsugamushi.
Results O. tsutsugamushi was detected in the case's blood samples by quantitative real-time fluorescent PCR. Phylogenetic tree analysis demonstrated it had most closely relationship with Boryong strain.
Conclusion An infection case of O. tsutsugamushi was laboratory confirmed in Ningbo, the sub-genotype was Boryong.

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