Primary Analysis of Virulent Genes of 114 Strains EHEC O₁₅₇∶H7in Anhui Province

To analyze and study the virulent Characteristic and the pathogenesis of EHEC O157∶H7; and to test the virulent genes of EHEC O157∶H7 isolated in Anhui Province. Methods Using PCR to test the specific gene fragment of two virulent factors (SLT and hly) of EHEC O157∶H7; and using serology test and biochemical test to identify to biological character of the strains. Result 63.2% of EHEC O157∶H7 strains carried SLT and hly genes, among the animal original strains livestock's given the highest rate,the rate of poultry was the second.It showed that the patho-genicity of EHEC O157∶H7 was powerful.14.0% of the 114 isolated strains metabolized sorbitol in 24 hours,18.8% of the strains that metabolism sorbitol carried these genes,which was lower than that did not metabolize sorbitol(70.4%),it showed some relevance between the mutation of metabolism sorbitol and lack of virulent genes.