Application of real-time PCR TaqMan assay for rapid detection of Campylobacter jejuni from stool samples

ObjectiveTo establish real-time PCR TaqMan assay for the direct detection of ICampylobacter jejuni/I from stool samples. MethodsTwo pairs of specific primers and probes were designed by IhipO/I and ImapA/I genes respectively. On the basis of evaluation of sensitivity, specificity and reproducibility of two systems, real-time PCR was performed to identify DNA extracted from 45 stool samples of clinical diarrhea patients and conventional culture was conducted too. ResultsIC. jujeni/I strain could be detected by the 2 pairs of primers and probes with the detection limit of 10-20 cfu/ml. There was no cross-reactions with other enteric pathogens. Of 45 stool sample, 3 were positive by real-time PCR, and 2 of which were positive by conventional pure culture. ConclusionReal-time PCR TaqMan assay in this study was highly sensitive and specific, which could rapidly detect IC. jejuni/I from stool samples with high positive rate and in a shorter time.